CRISPR-U™ Gene Knockout Cell Line
Strategy
BCAP31 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BCAP31 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BCAP31 |
| Gene id | 10134 |
| Organism | Homo sapiens |
| Official full symbol | B cell receptor associated protein 31 |
| Gene type | protein-coding |
| Also known as | 6C6-AG, BAP31, CDM, DDCH, DXS1357E |
| Summary | This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BCAP31-207 | ENST00000458587.8 | 1789 | 313aa | Protein coding | CCDS48191 | P51572-2 | - | TSL:1, GENCODE basic, |
| BCAP31-213 | ENST00000647529.1 | 1433 | 246aa | Protein coding | CCDS14727 | P51572-1 | - | GENCODE basic, APPRIS P1, |
| BCAP31-214 | ENST00000672675.1 | 1350 | 246aa | Protein coding | CCDS14727 | P51572-1 | - | APPRIS P1, |
| BCAP31-201 | ENST00000345046.12 | 1284 | 246aa | Protein coding | CCDS14727 | P51572-1 | NM_001256447.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BCAP31-210 | ENST00000645377.1 | 1175 | 211aa | Protein coding | - | A0A2R8Y5M6 | - | GENCODE basic, |
| BCAP31-206 | ENST00000442093.5 | 793 | 188aa | Protein coding | - | C9J0M4 | - | CDS 3' incomplete, TSL:2, |
| BCAP31-202 | ENST00000416815.5 | 688 | 182aa | Protein coding | - | C9JSP1 | - | CDS 3' incomplete, TSL:5, |
| BCAP31-204 | ENST00000429550.5 | 684 | 187aa | Protein coding | - | C9JQ75 | - | CDS 3' incomplete, TSL:3, |
| BCAP31-203 | ENST00000423827.5 | 669 | 178aa | Protein coding | - | C9JMD7 | - | CDS 3' incomplete, TSL:5, |
| BCAP31-205 | ENST00000430088.1 | 570 | 138aa | Protein coding | - | C9JM14 | - | CDS 3' incomplete, TSL:2, |
| BCAP31-212 | ENST00000646514.1 | 1217 | No protein | Processed transcript | - | - | - | - |
| BCAP31-209 | ENST00000645006.1 | 893 | No protein | Retained intron | - | - | - | - |
| BCAP31-211 | ENST00000645802.1 | 573 | No protein | Retained intron | - | - | - | - |
| BCAP31-208 | ENST00000468947.1 | 409 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BCAP31 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BCAP31 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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