CRISPR-U™ Gene Knockout Cell Line Strategy

BCAP31 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BCAP31 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BCAP31
Gene id 10134
Organism Homo sapiens
Official full symbol B cell receptor associated protein 31
Gene type protein-coding
Also known as 6C6-AG, BAP31, CDM, DDCH, DXS1357E
Summary This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16.
Genomic regions Chromosome X
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BCAP31-207 ENST00000458587.8 1789 313aa Protein coding CCDS48191 P51572-2 - TSL:1, GENCODE basic,
BCAP31-213 ENST00000647529.1 1433 246aa Protein coding CCDS14727 P51572-1 - GENCODE basic, APPRIS P1,
BCAP31-214 ENST00000672675.1 1350 246aa Protein coding CCDS14727 P51572-1 - APPRIS P1,
BCAP31-201 ENST00000345046.12 1284 246aa Protein coding CCDS14727 P51572-1 NM_001256447.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BCAP31-210 ENST00000645377.1 1175 211aa Protein coding - A0A2R8Y5M6 - GENCODE basic,
BCAP31-206 ENST00000442093.5 793 188aa Protein coding - C9J0M4 - CDS 3' incomplete, TSL:2,
BCAP31-202 ENST00000416815.5 688 182aa Protein coding - C9JSP1 - CDS 3' incomplete, TSL:5,
BCAP31-204 ENST00000429550.5 684 187aa Protein coding - C9JQ75 - CDS 3' incomplete, TSL:3,
BCAP31-203 ENST00000423827.5 669 178aa Protein coding - C9JMD7 - CDS 3' incomplete, TSL:5,
BCAP31-205 ENST00000430088.1 570 138aa Protein coding - C9JM14 - CDS 3' incomplete, TSL:2,
BCAP31-212 ENST00000646514.1 1217 No protein Processed transcript - - - -
BCAP31-209 ENST00000645006.1 893 No protein Retained intron - - - -
BCAP31-211 ENST00000645802.1 573 No protein Retained intron - - - -
BCAP31-208 ENST00000468947.1 409 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BCAP31
This KO Strategy loading
Red Cotton™ Notes Gene BCAP31 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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