CRISPR-U™ Gene Knockout Cell Line Strategy

PTEN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTEN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTEN
Gene id 5728
Organism Homo sapiens
Official full symbol phosphatase and tensin homolog
Gene type protein-coding
Also known as 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1
Summary This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 10
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTEN-201 ENST00000371953.8 8515 403aa Protein coding CCDS31238 P60484-1 NM_000314.8 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PTEN-203 ENST00000472832.2 644 153aa Protein coding - A0A087X033 - CDS 5' incomplete, TSL:2,
PTEN-206 ENST00000618586.1 1581 No protein Processed transcript - - - TSL:NA,
PTEN-204 ENST00000487939.1 477 No protein Processed transcript - - - TSL:3,
PTEN-205 ENST00000498703.1 554 No protein Retained intron - - - TSL:2,
PTEN-202 ENST00000462694.1 383 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTEN
This KO Strategy loading
Red Cotton™ Notes Gene PTEN had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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