CRISPR-U™ Gene Knockout Cell Line
Strategy
CD47 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CD47 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CD47 |
| Gene id | 961 |
| Organism | Homo sapiens |
| Official full symbol | CD47 molecule |
| Gene type | protein-coding |
| Also known as | IAP, MER6, OA3 |
| Summary | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CD47-202 | ENST00000361309.6 | 5292 | 323aa | Protein coding | CCDS43126 | Q08722-1 | NM_001777.4 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| CD47-201 | ENST00000355354.13 | 5228 | 305aa | Protein coding | CCDS43125 | Q08722-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CD47-206 | ENST00000644850.1 | 766 | 183aa | Protein coding | - | A0A2R8Y484 | - | CDS 3' incomplete, |
| CD47-205 | ENST00000517766.5 | 538 | 109aa | Protein coding | - | H0YC40 | - | CDS 5' incomplete, TSL:2, |
| CD47-203 | ENST00000398258.7 | 469 | 86aa | Protein coding | - | H7BYS8 | - | CDS 5' incomplete, TSL:3, |
| CD47-204 | ENST00000471694.1 | 6796 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CD47 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CD47 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: