CRISPR-U™ Gene Knockout Cell Line Strategy

CD47 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CD47 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CD47
Gene id 961
Organism Homo sapiens
Official full symbol CD47 molecule
Gene type protein-coding
Also known as IAP, MER6, OA3
Summary This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
Genomic regions Chromosome 3
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CD47-202 ENST00000361309.6 5292 323aa Protein coding CCDS43126 Q08722-1 NM_001777.4 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
CD47-201 ENST00000355354.13 5228 305aa Protein coding CCDS43125 Q08722-3 - TSL:1, GENCODE basic, APPRIS ALT2,
CD47-206 ENST00000644850.1 766 183aa Protein coding - A0A2R8Y484 - CDS 3' incomplete,
CD47-205 ENST00000517766.5 538 109aa Protein coding - H0YC40 - CDS 5' incomplete, TSL:2,
CD47-203 ENST00000398258.7 469 86aa Protein coding - H7BYS8 - CDS 5' incomplete, TSL:3,
CD47-204 ENST00000471694.1 6796 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CD47
This KO Strategy loading
Red Cotton™ Notes Gene CD47 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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