CRISPR-U™ Gene Knockout Cell Line
Strategy
BSG Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BSG Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BSG |
| Gene id | 682 |
| Organism | Homo sapiens |
| Official full symbol | basigin (Ok blood group) |
| Gene type | protein-coding |
| Also known as | 5F7, CD147, EMMPRIN, EMPRIN, OK, SLC7A11, TCSF |
| Summary | The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 13 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BSG-201 | ENST00000333511.9 | 1946 | 385aa | Protein coding | CCDS12033 | P35613-1 | NM_001728.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BSG-204 | ENST00000545507.6 | 1795 | 176aa | Protein coding | CCDS58635 | P35613-3 | - | TSL:1, GENCODE basic, |
| BSG-211 | ENST00000576984.3 | 1666 | 176aa | Protein coding | CCDS58635 | R4GN83 | - | TSL:4, GENCODE basic, |
| BSG-218 | ENST00000680065.1 | 1630 | 176aa | Protein coding | CCDS58635 | - | - | GENCODE basic, |
| BSG-202 | ENST00000346916.9 | 1605 | 176aa | Protein coding | CCDS58635 | P35613-4 | - | TSL:1, GENCODE basic, |
| BSG-203 | ENST00000353555.9 | 1598 | 269aa | Protein coding | CCDS12034 | P35613-2 | - | TSL:1, GENCODE basic, |
| BSG-208 | ENST00000573784.6 | 1587 | 176aa | Protein coding | CCDS58635 | R4GMX5 | - | TSL:4, GENCODE basic, |
| BSG-217 | ENST00000679472.1 | 1610 | 206aa | Protein coding | - | - | - | GENCODE basic, |
| BSG-221 | ENST00000680552.1 | 1605 | 266aa | Protein coding | - | - | - | GENCODE basic, |
| BSG-219 | ENST00000680326.1 | 1575 | 266aa | Protein coding | - | - | - | GENCODE basic, |
| BSG-215 | ENST00000618006.4 | 1329 | 189aa | Protein coding | - | A0A087WUV8 | - | TSL:2, GENCODE basic, |
| BSG-214 | ENST00000614867.2 | 960 | 55aa | Protein coding | - | A0A087X215 | - | TSL:3, GENCODE basic, |
| BSG-207 | ENST00000573216.5 | 545 | 160aa | Protein coding | - | I3L192 | - | CDS 3' incomplete, TSL:4, |
| BSG-216 | ENST00000618112.4 | 1519 | 103aa | Nonsense mediated decay | - | A0A2R8YDZ0 | - | TSL:2, |
| BSG-213 | ENST00000613627.5 | 579 | 54aa | Nonsense mediated decay | - | A0A087X2B5 | - | TSL:3, |
| BSG-209 | ENST00000574970.2 | 570 | No protein | Processed transcript | - | - | - | TSL:4, |
| BSG-210 | ENST00000576925.4 | 2314 | No protein | Retained intron | - | - | - | TSL:2, |
| BSG-205 | ENST00000571735.3 | 2141 | No protein | Retained intron | - | - | - | TSL:2, |
| BSG-206 | ENST00000572899.6 | 946 | No protein | Retained intron | - | - | - | TSL:2, |
| BSG-220 | ENST00000680551.1 | 693 | No protein | Retained intron | - | - | - | - |
| BSG-212 | ENST00000590218.4 | 545 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BSG |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BSG had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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