CRISPR-U™ Gene Knockout Cell Line Strategy

BSG Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BSG Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BSG
Gene id 682
Organism Homo sapiens
Official full symbol basigin (Ok blood group)
Gene type protein-coding
Also known as 5F7, CD147, EMMPRIN, EMPRIN, OK, SLC7A11, TCSF
Summary The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BSG-201 ENST00000333511.9 1946 385aa Protein coding CCDS12033 P35613-1 NM_001728.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BSG-204 ENST00000545507.6 1795 176aa Protein coding CCDS58635 P35613-3 - TSL:1, GENCODE basic,
BSG-211 ENST00000576984.3 1666 176aa Protein coding CCDS58635 R4GN83 - TSL:4, GENCODE basic,
BSG-218 ENST00000680065.1 1630 176aa Protein coding CCDS58635 - - GENCODE basic,
BSG-202 ENST00000346916.9 1605 176aa Protein coding CCDS58635 P35613-4 - TSL:1, GENCODE basic,
BSG-203 ENST00000353555.9 1598 269aa Protein coding CCDS12034 P35613-2 - TSL:1, GENCODE basic,
BSG-208 ENST00000573784.6 1587 176aa Protein coding CCDS58635 R4GMX5 - TSL:4, GENCODE basic,
BSG-217 ENST00000679472.1 1610 206aa Protein coding - - - GENCODE basic,
BSG-221 ENST00000680552.1 1605 266aa Protein coding - - - GENCODE basic,
BSG-219 ENST00000680326.1 1575 266aa Protein coding - - - GENCODE basic,
BSG-215 ENST00000618006.4 1329 189aa Protein coding - A0A087WUV8 - TSL:2, GENCODE basic,
BSG-214 ENST00000614867.2 960 55aa Protein coding - A0A087X215 - TSL:3, GENCODE basic,
BSG-207 ENST00000573216.5 545 160aa Protein coding - I3L192 - CDS 3' incomplete, TSL:4,
BSG-216 ENST00000618112.4 1519 103aa Nonsense mediated decay - A0A2R8YDZ0 - TSL:2,
BSG-213 ENST00000613627.5 579 54aa Nonsense mediated decay - A0A087X2B5 - TSL:3,
BSG-209 ENST00000574970.2 570 No protein Processed transcript - - - TSL:4,
BSG-210 ENST00000576925.4 2314 No protein Retained intron - - - TSL:2,
BSG-205 ENST00000571735.3 2141 No protein Retained intron - - - TSL:2,
BSG-206 ENST00000572899.6 946 No protein Retained intron - - - TSL:2,
BSG-220 ENST00000680551.1 693 No protein Retained intron - - - -
BSG-212 ENST00000590218.4 545 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BSG
This KO Strategy loading
Red Cotton™ Notes Gene BSG had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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