CRISPR-U™ Gene Knockout Cell Line
Strategy
DAZAP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DAZAP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DAZAP1 |
| Gene id | 26528 |
| Organism | Homo sapiens |
| Official full symbol | DAZ associated protein 1 |
| Gene type | protein-coding |
| Summary | In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DAZAP1-202 | ENST00000336761.10 | 2290 | 378aa | Protein coding | CCDS12066 | Q96EP5-2 | - | TSL:5, GENCODE basic, |
| DAZAP1-201 | ENST00000233078.9 | 2184 | 407aa | Protein coding | CCDS12065 | Q96EP5-1 | NM_018959.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| DAZAP1-211 | ENST00000592522.5 | 2157 | 406aa | Protein coding | CCDS86684 | K7EQ55 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| DAZAP1-205 | ENST00000587079.5 | 2086 | 406aa | Protein coding | CCDS86685 | K7EK33 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| DAZAP1-210 | ENST00000592453.2 | 1021 | 327aa | Protein coding | - | K7EQ02 | - | CDS 3' incomplete, TSL:5, |
| DAZAP1-204 | ENST00000586579.5 | 619 | No protein | Processed transcript | - | - | - | TSL:3, |
| DAZAP1-206 | ENST00000587833.5 | 560 | No protein | Processed transcript | - | - | - | TSL:5, |
| DAZAP1-209 | ENST00000590419.5 | 508 | No protein | Processed transcript | - | - | - | TSL:3, |
| DAZAP1-203 | ENST00000585485.2 | 3943 | No protein | Retained intron | - | - | - | TSL:2, |
| DAZAP1-208 | ENST00000589874.2 | 3642 | No protein | Retained intron | - | - | - | TSL:NA, |
| DAZAP1-207 | ENST00000589484.5 | 3414 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DAZAP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DAZAP1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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