CRISPR-U™ Gene Knockout Cell Line Strategy

DAZAP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DAZAP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DAZAP1
Gene id 26528
Organism Homo sapiens
Official full symbol DAZ associated protein 1
Gene type protein-coding
Summary In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DAZAP1-202 ENST00000336761.10 2290 378aa Protein coding CCDS12066 Q96EP5-2 - TSL:5, GENCODE basic,
DAZAP1-201 ENST00000233078.9 2184 407aa Protein coding CCDS12065 Q96EP5-1 NM_018959.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
DAZAP1-211 ENST00000592522.5 2157 406aa Protein coding CCDS86684 K7EQ55 - TSL:5, GENCODE basic, APPRIS ALT1,
DAZAP1-205 ENST00000587079.5 2086 406aa Protein coding CCDS86685 K7EK33 - TSL:5, GENCODE basic, APPRIS ALT1,
DAZAP1-210 ENST00000592453.2 1021 327aa Protein coding - K7EQ02 - CDS 3' incomplete, TSL:5,
DAZAP1-204 ENST00000586579.5 619 No protein Processed transcript - - - TSL:3,
DAZAP1-206 ENST00000587833.5 560 No protein Processed transcript - - - TSL:5,
DAZAP1-209 ENST00000590419.5 508 No protein Processed transcript - - - TSL:3,
DAZAP1-203 ENST00000585485.2 3943 No protein Retained intron - - - TSL:2,
DAZAP1-208 ENST00000589874.2 3642 No protein Retained intron - - - TSL:NA,
DAZAP1-207 ENST00000589484.5 3414 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DAZAP1
This KO Strategy loading
Red Cotton™ Notes Gene DAZAP1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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