CRISPR-U™ Gene Knockout Cell Line Strategy

EIF2AK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EIF2AK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EIF2AK2
Gene id 5610
Organism Homo sapiens
Official full symbol eukaryotic translation initiation factor 2 alpha kinase 2
Gene type protein-coding
Also known as EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR
Summary The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EIF2AK2-213 ENST00000681507.1 10103 551aa Protein coding CCDS1786 - - APPRIS P2,
EIF2AK2-201 ENST00000233057.9 9975 551aa Protein coding CCDS1786 P19525-1 NM_001135651.3 TSL:2, GENCODE basic, APPRIS P2, MANE Select v0.92,
EIF2AK2-212 ENST00000681463.1 9931 551aa Protein coding CCDS1786 - - APPRIS P2,
EIF2AK2-208 ENST00000679507.1 9835 551aa Protein coding CCDS1786 - - GENCODE basic, APPRIS P2,
EIF2AK2-202 ENST00000395127.6 4343 551aa Protein coding CCDS1786 P19525-1 - TSL:5, GENCODE basic, APPRIS P2,
EIF2AK2-203 ENST00000405334.5 1533 510aa Protein coding CCDS46259 P19525-2 - TSL:1, GENCODE basic,
EIF2AK2-209 ENST00000679979.1 9846 508aa Protein coding - - - GENCODE basic,
EIF2AK2-214 ENST00000681516.1 9837 541aa Protein coding - - - GENCODE basic,
EIF2AK2-210 ENST00000680273.1 9766 494aa Protein coding - - - GENCODE basic, APPRIS ALT2,
EIF2AK2-207 ENST00000647926.1 2235 551aa Nonsense mediated decay CCDS1786 P19525-1 - -
EIF2AK2-211 ENST00000681329.1 10058 No protein Processed transcript - - - -
EIF2AK2-204 ENST00000411537.7 1272 No protein Processed transcript - - - TSL:5,
EIF2AK2-206 ENST00000496059.1 845 No protein Retained intron - - - TSL:3,
EIF2AK2-205 ENST00000462861.1 411 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EIF2AK2
This KO Strategy loading
Red Cotton™ Notes Gene EIF2AK2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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