CRISPR-U™ Gene Knockout Cell Line
Strategy
EIF2AK2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EIF2AK2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EIF2AK2 |
| Gene id | 5610 |
| Organism | Homo sapiens |
| Official full symbol | eukaryotic translation initiation factor 2 alpha kinase 2 |
| Gene type | protein-coding |
| Also known as | EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR |
| Summary | The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EIF2AK2-213 | ENST00000681507.1 | 10103 | 551aa | Protein coding | CCDS1786 | - | - | APPRIS P2, |
| EIF2AK2-201 | ENST00000233057.9 | 9975 | 551aa | Protein coding | CCDS1786 | P19525-1 | NM_001135651.3 | TSL:2, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| EIF2AK2-212 | ENST00000681463.1 | 9931 | 551aa | Protein coding | CCDS1786 | - | - | APPRIS P2, |
| EIF2AK2-208 | ENST00000679507.1 | 9835 | 551aa | Protein coding | CCDS1786 | - | - | GENCODE basic, APPRIS P2, |
| EIF2AK2-202 | ENST00000395127.6 | 4343 | 551aa | Protein coding | CCDS1786 | P19525-1 | - | TSL:5, GENCODE basic, APPRIS P2, |
| EIF2AK2-203 | ENST00000405334.5 | 1533 | 510aa | Protein coding | CCDS46259 | P19525-2 | - | TSL:1, GENCODE basic, |
| EIF2AK2-209 | ENST00000679979.1 | 9846 | 508aa | Protein coding | - | - | - | GENCODE basic, |
| EIF2AK2-214 | ENST00000681516.1 | 9837 | 541aa | Protein coding | - | - | - | GENCODE basic, |
| EIF2AK2-210 | ENST00000680273.1 | 9766 | 494aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| EIF2AK2-207 | ENST00000647926.1 | 2235 | 551aa | Nonsense mediated decay | CCDS1786 | P19525-1 | - | - |
| EIF2AK2-211 | ENST00000681329.1 | 10058 | No protein | Processed transcript | - | - | - | - |
| EIF2AK2-204 | ENST00000411537.7 | 1272 | No protein | Processed transcript | - | - | - | TSL:5, |
| EIF2AK2-206 | ENST00000496059.1 | 845 | No protein | Retained intron | - | - | - | TSL:3, |
| EIF2AK2-205 | ENST00000462861.1 | 411 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EIF2AK2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EIF2AK2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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