CRISPR-U™ Gene Knockout Cell Line Strategy

NFKB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NFKB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NFKB1
Gene id 4790
Organism Homo sapiens
Official full symbol nuclear factor kappa B subunit 1
Gene type protein-coding
Also known as CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB
Summary This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
Genomic regions Chromosome 4
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NFKB1-201 ENST00000226574.9 4055 969aa Protein coding CCDS3657 P19838-2 NM_003998.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
NFKB1-205 ENST00000505458.5 3707 968aa Protein coding CCDS54783 P19838-1 - TSL:1, GENCODE basic, APPRIS ALT2,
NFKB1-202 ENST00000394820.8 3693 968aa Protein coding CCDS54783 P19838-1 - TSL:1, GENCODE basic, APPRIS ALT2,
NFKB1-212 ENST00000600343.5 4010 788aa Protein coding - P19838-3 - TSL:2, GENCODE basic,
NFKB1-206 ENST00000507079.5 965 198aa Protein coding - D6RH30 - CDS 3' incomplete, TSL:5,
NFKB1-207 ENST00000508584.1 751 196aa Protein coding - D6RF93 - CDS 3' incomplete, TSL:3,
NFKB1-210 ENST00000511926.5 609 74aa Protein coding - D6RDI9 - CDS 3' incomplete, TSL:5,
NFKB1-208 ENST00000509165.1 557 144aa Protein coding - D6RC45 - CDS 3' incomplete, TSL:5,
NFKB1-214 ENST00000652619.1 4115 511aa Nonsense mediated decay - A0A494C1E9 - -
NFKB1-213 ENST00000652569.1 3594 253aa Nonsense mediated decay - A0A494C157 - CDS 5' incomplete,
NFKB1-209 ENST00000510638.1 628 No protein Processed transcript - - - TSL:4,
NFKB1-211 ENST00000513803.5 612 No protein Processed transcript - - - TSL:4,
NFKB1-204 ENST00000504044.1 580 No protein Retained intron - - - TSL:2,
NFKB1-203 ENST00000502367.1 456 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NFKB1
This KO Strategy loading
Red Cotton™ Notes Gene NFKB1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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