CRISPR-U™ Gene Knockout Cell Line
Strategy
NFKB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NFKB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NFKB1 |
| Gene id | 4790 |
| Organism | Homo sapiens |
| Official full symbol | nuclear factor kappa B subunit 1 |
| Gene type | protein-coding |
| Also known as | CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB |
| Summary | This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NFKB1-201 | ENST00000226574.9 | 4055 | 969aa | Protein coding | CCDS3657 | P19838-2 | NM_003998.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| NFKB1-205 | ENST00000505458.5 | 3707 | 968aa | Protein coding | CCDS54783 | P19838-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NFKB1-202 | ENST00000394820.8 | 3693 | 968aa | Protein coding | CCDS54783 | P19838-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NFKB1-212 | ENST00000600343.5 | 4010 | 788aa | Protein coding | - | P19838-3 | - | TSL:2, GENCODE basic, |
| NFKB1-206 | ENST00000507079.5 | 965 | 198aa | Protein coding | - | D6RH30 | - | CDS 3' incomplete, TSL:5, |
| NFKB1-207 | ENST00000508584.1 | 751 | 196aa | Protein coding | - | D6RF93 | - | CDS 3' incomplete, TSL:3, |
| NFKB1-210 | ENST00000511926.5 | 609 | 74aa | Protein coding | - | D6RDI9 | - | CDS 3' incomplete, TSL:5, |
| NFKB1-208 | ENST00000509165.1 | 557 | 144aa | Protein coding | - | D6RC45 | - | CDS 3' incomplete, TSL:5, |
| NFKB1-214 | ENST00000652619.1 | 4115 | 511aa | Nonsense mediated decay | - | A0A494C1E9 | - | - |
| NFKB1-213 | ENST00000652569.1 | 3594 | 253aa | Nonsense mediated decay | - | A0A494C157 | - | CDS 5' incomplete, |
| NFKB1-209 | ENST00000510638.1 | 628 | No protein | Processed transcript | - | - | - | TSL:4, |
| NFKB1-211 | ENST00000513803.5 | 612 | No protein | Processed transcript | - | - | - | TSL:4, |
| NFKB1-204 | ENST00000504044.1 | 580 | No protein | Retained intron | - | - | - | TSL:2, |
| NFKB1-203 | ENST00000502367.1 | 456 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NFKB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NFKB1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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