CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ACE2 Knockout
model in NCI-H520 cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info
Official symbol
ACE2
Gene id
59272
Organism
Homo sapiens
Official full symbol
angiotensin I converting enzyme 2
Gene type
protein-coding
Also known as
ACEH
Summary
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the causative agent of coronavirus disease-2019 (COVID-19).
Genomic regions
Chromosome X
Cell line info
Cell name
NCI-H520
Tissue
lung
Organism
Human
Morphology
epithelial
Culture properties
adherent
Disease
squamous cell carcinoma
Derivation
The NCI-H520 cell line was derived by A.F. Gazdar and associates in 1982 from a sample of a lung mass taken from a patient with squamous cell carcimoma of the lung.
Complete growth medium
Complete Growth MediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: · fetal bovine serum to a final concentration of 10%This medium is formulated for use with a 5% CO2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020 (500 mL) and ATCC Catalog No. 30-2021 (100 mL).
Subcultivation ratio
A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Strategy Summary
This gene has 8 protein coding transcripts:
Name
Transcript ID
bp
Protein
Biotype
CCDS
UniProt Match
RefSeq Match
Flags
ACE2-202
ENST00000427411.2
7587
805aa
Protein coding
CCDS14169
Q9BYF1-1
-
TSL:1, GENCODE basic, APPRIS P2,
ACE2-207
ENST00000678073.1
7487
805aa
Protein coding
CCDS14169
-
-
GENCODE basic, APPRIS P2,
ACE2-211
ENST00000680121.1
7413
805aa
Protein coding
CCDS14169
-
-
GENCODE basic, APPRIS P2,
ACE2-201
ENST00000252519.8
3339
805aa
Protein coding
CCDS14169
Q9BYF1-1
NM_001371415.1
TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
Ubigene has successfully modified
NCI-H520
cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.