CRISPR-U™ Gene Knockout Cell Line
Strategy
HK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HK1 |
| Gene id | 3098 |
| Organism | Homo sapiens |
| Official full symbol | hexokinase 1 |
| Gene type | protein-coding |
| Also known as | HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, RP79, hexokinase |
| Summary | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HK1-218 | ENST00000643399.2 | 3998 | 921aa | Protein coding | CCDS7289 | P19367-3 | NM_001358263.1 | GENCODE basic, MANE Plus Clinical v0.92, |
| HK1-206 | ENST00000448642.6 | 3868 | 921aa | Protein coding | CCDS7289 | P19367-3 | - | TSL:5, GENCODE basic, |
| HK1-205 | ENST00000436817.6 | 3835 | 921aa | Protein coding | CCDS7289 | P19367-3 | - | TSL:5, GENCODE basic, |
| HK1-202 | ENST00000359426.7 | 3602 | 917aa | Protein coding | CCDS7292 | P19367-1 | NM_000188.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HK1-201 | ENST00000298649.8 | 3229 | 916aa | Protein coding | CCDS7291 | P19367-2 | - | TSL:5, GENCODE basic, |
| HK1-203 | ENST00000360289.6 | 3961 | 905aa | Protein coding | - | P19367-4 | - | TSL:5, GENCODE basic, |
| HK1-204 | ENST00000421088.5 | 707 | 114aa | Protein coding | - | B1AR62 | - | CDS 3' incomplete, TSL:5, |
| HK1-208 | ENST00000464803.6 | 567 | 59aa | Protein coding | - | A0A2R8Y7T9 | - | CDS 3' incomplete, TSL:1, |
| HK1-210 | ENST00000476368.6 | 456 | 29aa | Protein coding | - | A0A2R8YEE7 | - | CDS 3' incomplete, TSL:3, |
| HK1-207 | ENST00000450646.6 | 449 | 30aa | Protein coding | - | P78542 | - | CDS 3' incomplete, TSL:5, |
| HK1-216 | ENST00000493591.6 | 3745 | 92aa | Nonsense mediated decay | - | A0A2R8Y626 | - | TSL:5, |
| HK1-214 | ENST00000483077.5 | 750 | No protein | Processed transcript | - | - | - | TSL:3, |
| HK1-211 | ENST00000479594.5 | 680 | No protein | Processed transcript | - | - | - | TSL:3, |
| HK1-215 | ENST00000488644.5 | 593 | No protein | Processed transcript | - | - | - | TSL:2, |
| HK1-213 | ENST00000483054.5 | 592 | No protein | Processed transcript | - | - | - | TSL:3, |
| HK1-212 | ENST00000480047.5 | 575 | No protein | Processed transcript | - | - | - | TSL:1, |
| HK1-209 | ENST00000470050.1 | 314 | No protein | Processed transcript | - | - | - | TSL:5, |
| HK1-217 | ENST00000494253.1 | 2848 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HK1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: