CRISPR-U™ Gene Knockout Cell Line Strategy

HK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HK1
Gene id 3098
Organism Homo sapiens
Official full symbol hexokinase 1
Gene type protein-coding
Also known as HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, RP79, hexokinase
Summary Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Genomic regions Chromosome 10
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HK1-218 ENST00000643399.2 3998 921aa Protein coding CCDS7289 P19367-3 NM_001358263.1 GENCODE basic, MANE Plus Clinical v0.92,
HK1-206 ENST00000448642.6 3868 921aa Protein coding CCDS7289 P19367-3 - TSL:5, GENCODE basic,
HK1-205 ENST00000436817.6 3835 921aa Protein coding CCDS7289 P19367-3 - TSL:5, GENCODE basic,
HK1-202 ENST00000359426.7 3602 917aa Protein coding CCDS7292 P19367-1 NM_000188.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HK1-201 ENST00000298649.8 3229 916aa Protein coding CCDS7291 P19367-2 - TSL:5, GENCODE basic,
HK1-203 ENST00000360289.6 3961 905aa Protein coding - P19367-4 - TSL:5, GENCODE basic,
HK1-204 ENST00000421088.5 707 114aa Protein coding - B1AR62 - CDS 3' incomplete, TSL:5,
HK1-208 ENST00000464803.6 567 59aa Protein coding - A0A2R8Y7T9 - CDS 3' incomplete, TSL:1,
HK1-210 ENST00000476368.6 456 29aa Protein coding - A0A2R8YEE7 - CDS 3' incomplete, TSL:3,
HK1-207 ENST00000450646.6 449 30aa Protein coding - P78542 - CDS 3' incomplete, TSL:5,
HK1-216 ENST00000493591.6 3745 92aa Nonsense mediated decay - A0A2R8Y626 - TSL:5,
HK1-214 ENST00000483077.5 750 No protein Processed transcript - - - TSL:3,
HK1-211 ENST00000479594.5 680 No protein Processed transcript - - - TSL:3,
HK1-215 ENST00000488644.5 593 No protein Processed transcript - - - TSL:2,
HK1-213 ENST00000483054.5 592 No protein Processed transcript - - - TSL:3,
HK1-212 ENST00000480047.5 575 No protein Processed transcript - - - TSL:1,
HK1-209 ENST00000470050.1 314 No protein Processed transcript - - - TSL:5,
HK1-217 ENST00000494253.1 2848 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HK1
This KO Strategy loading
Red Cotton™ Notes Gene HK1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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