CRISPR-U™ Gene Knockout Cell Line Strategy

CELF1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CELF1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CELF1
Gene id 10658
Organism Homo sapiens
Official full symbol CUGBP Elav-like family member 1
Gene type protein-coding
Also known as BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, NAB50, NAPOR, hNab50
Summary Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 11
Strategy Summary
This gene has 15 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CELF1-204 ENST00000395290.6 7666 485aa Protein coding CCDS53622 Q92879-6 - TSL:2, GENCODE basic,
CELF1-201 ENST00000310513.10 4583 482aa Protein coding CCDS7938 Q92879-2 - TSL:1, GENCODE basic, APPRIS ALT1,
CELF1-214 ENST00000531165.5 3650 514aa Protein coding CCDS81565 G5EA30 - TSL:2, GENCODE basic, APPRIS P3,
CELF1-215 ENST00000532048.5 3044 512aa Protein coding CCDS53623 Q92879-4 - TSL:1, GENCODE basic, APPRIS ALT1,
CELF1-205 ENST00000395292.6 2191 483aa Protein coding CCDS7939 Q92879-3 - TSL:1, GENCODE basic,
CELF1-202 ENST00000358597.7 2108 486aa Protein coding CCDS31482 Q92879-1 - TSL:1, GENCODE basic, APPRIS ALT1,
CELF1-203 ENST00000361904.7 1580 483aa Protein coding CCDS7939 Q92879-3 - TSL:1, GENCODE basic,
CELF1-208 ENST00000525841.5 622 87aa Protein coding - E9PQK4 - CDS 3' incomplete, TSL:5,
CELF1-213 ENST00000530151.5 583 95aa Protein coding - E9PSH0 - CDS 3' incomplete, TSL:3,
CELF1-212 ENST00000528538.5 581 14aa Protein coding - F5H7M7 - CDS 3' incomplete, TSL:4,
CELF1-221 ENST00000543178.5 576 78aa Protein coding - F5H4Y5 - CDS 3' incomplete, TSL:4,
CELF1-209 ENST00000526277.1 556 37aa Protein coding - F5H3J7 - CDS 3' incomplete, TSL:4,
CELF1-218 ENST00000535982.1 549 103aa Protein coding - F5H0D8 - CDS 3' incomplete, TSL:4,
CELF1-211 ENST00000528434.5 543 114aa Protein coding - E9PKU1 - CDS 3' incomplete, TSL:5,
CELF1-210 ENST00000526419.1 476 56aa Protein coding - E9PKA1 - CDS 3' incomplete, TSL:4,
CELF1-220 ENST00000539455.5 800 No protein Processed transcript - - - TSL:5,
CELF1-219 ENST00000539254.1 572 No protein Processed transcript - - - TSL:4,
CELF1-206 ENST00000422993.2 553 No protein Processed transcript - - - TSL:5,
CELF1-207 ENST00000524648.1 393 No protein Processed transcript - - - TSL:2,
CELF1-217 ENST00000534614.1 567 No protein Retained intron - - - TSL:4,
CELF1-216 ENST00000532146.1 473 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CELF1
This KO Strategy loading
Red Cotton™ Notes Gene CELF1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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