CRISPR-U™ Gene Knockout Cell Line
Strategy
CELF1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CELF1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CELF1 |
| Gene id | 10658 |
| Organism | Homo sapiens |
| Official full symbol | CUGBP Elav-like family member 1 |
| Gene type | protein-coding |
| Also known as | BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, NAB50, NAPOR, hNab50 |
| Summary | Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 15 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CELF1-204 | ENST00000395290.6 | 7666 | 485aa | Protein coding | CCDS53622 | Q92879-6 | - | TSL:2, GENCODE basic, |
| CELF1-201 | ENST00000310513.10 | 4583 | 482aa | Protein coding | CCDS7938 | Q92879-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| CELF1-214 | ENST00000531165.5 | 3650 | 514aa | Protein coding | CCDS81565 | G5EA30 | - | TSL:2, GENCODE basic, APPRIS P3, |
| CELF1-215 | ENST00000532048.5 | 3044 | 512aa | Protein coding | CCDS53623 | Q92879-4 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| CELF1-205 | ENST00000395292.6 | 2191 | 483aa | Protein coding | CCDS7939 | Q92879-3 | - | TSL:1, GENCODE basic, |
| CELF1-202 | ENST00000358597.7 | 2108 | 486aa | Protein coding | CCDS31482 | Q92879-1 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| CELF1-203 | ENST00000361904.7 | 1580 | 483aa | Protein coding | CCDS7939 | Q92879-3 | - | TSL:1, GENCODE basic, |
| CELF1-208 | ENST00000525841.5 | 622 | 87aa | Protein coding | - | E9PQK4 | - | CDS 3' incomplete, TSL:5, |
| CELF1-213 | ENST00000530151.5 | 583 | 95aa | Protein coding | - | E9PSH0 | - | CDS 3' incomplete, TSL:3, |
| CELF1-212 | ENST00000528538.5 | 581 | 14aa | Protein coding | - | F5H7M7 | - | CDS 3' incomplete, TSL:4, |
| CELF1-221 | ENST00000543178.5 | 576 | 78aa | Protein coding | - | F5H4Y5 | - | CDS 3' incomplete, TSL:4, |
| CELF1-209 | ENST00000526277.1 | 556 | 37aa | Protein coding | - | F5H3J7 | - | CDS 3' incomplete, TSL:4, |
| CELF1-218 | ENST00000535982.1 | 549 | 103aa | Protein coding | - | F5H0D8 | - | CDS 3' incomplete, TSL:4, |
| CELF1-211 | ENST00000528434.5 | 543 | 114aa | Protein coding | - | E9PKU1 | - | CDS 3' incomplete, TSL:5, |
| CELF1-210 | ENST00000526419.1 | 476 | 56aa | Protein coding | - | E9PKA1 | - | CDS 3' incomplete, TSL:4, |
| CELF1-220 | ENST00000539455.5 | 800 | No protein | Processed transcript | - | - | - | TSL:5, |
| CELF1-219 | ENST00000539254.1 | 572 | No protein | Processed transcript | - | - | - | TSL:4, |
| CELF1-206 | ENST00000422993.2 | 553 | No protein | Processed transcript | - | - | - | TSL:5, |
| CELF1-207 | ENST00000524648.1 | 393 | No protein | Processed transcript | - | - | - | TSL:2, |
| CELF1-217 | ENST00000534614.1 | 567 | No protein | Retained intron | - | - | - | TSL:4, |
| CELF1-216 | ENST00000532146.1 | 473 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CELF1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CELF1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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