CRISPR-U™ Gene Knockout Cell Line Strategy

FOLR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FOLR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FOLR1
Gene id 2348
Organism Homo sapiens
Official full symbol folate receptor alpha
Gene type protein-coding
Also known as FBP, FOLR, FRalpha
Summary The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FOLR1-203 ENST00000393679.5 1331 257aa Protein coding CCDS8211 A0A024R5H1 P15328 - TSL:1, GENCODE basic, APPRIS P1,
FOLR1-201 ENST00000312293.9 1130 257aa Protein coding CCDS8211 A0A024R5H1 P15328 - TSL:1, GENCODE basic, APPRIS P1,
FOLR1-204 ENST00000393681.6 1061 257aa Protein coding CCDS8211 A0A024R5H1 P15328 - TSL:1, GENCODE basic, APPRIS P1,
FOLR1-205 ENST00000675784.1 945 257aa Protein coding CCDS8211 A0A024R5H1 - GENCODE basic, APPRIS P1,
FOLR1-202 ENST00000393676.5 930 257aa Protein coding CCDS8211 A0A024R5H1 P15328 NM_016729.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FOLR1
This KO Strategy loading
Red Cotton™ Notes Gene FOLR1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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