CRISPR-U™ Gene Knockout Cell Line
Strategy
FOLR1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FOLR1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FOLR1 |
| Gene id | 2348 |
| Organism | Homo sapiens |
| Official full symbol | folate receptor alpha |
| Gene type | protein-coding |
| Also known as | FBP, FOLR, FRalpha |
| Summary | The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FOLR1-203 | ENST00000393679.5 | 1331 | 257aa | Protein coding | CCDS8211 | A0A024R5H1 P15328 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FOLR1-201 | ENST00000312293.9 | 1130 | 257aa | Protein coding | CCDS8211 | A0A024R5H1 P15328 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FOLR1-204 | ENST00000393681.6 | 1061 | 257aa | Protein coding | CCDS8211 | A0A024R5H1 P15328 | - | TSL:1, GENCODE basic, APPRIS P1, |
| FOLR1-205 | ENST00000675784.1 | 945 | 257aa | Protein coding | CCDS8211 | A0A024R5H1 | - | GENCODE basic, APPRIS P1, |
| FOLR1-202 | ENST00000393676.5 | 930 | 257aa | Protein coding | CCDS8211 | A0A024R5H1 P15328 | NM_016729.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FOLR1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FOLR1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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