CRISPR-U™ Gene Knockout Cell Line Strategy

ITGB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ITGB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ITGB1
Gene id 3688
Organism Homo sapiens
Official full symbol integrin subunit beta 1
Gene type protein-coding
Also known as CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB
Summary Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
Genomic regions Chromosome 10
Strategy Summary
This gene has 26 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ITGB1-206 ENST00000437302.6 4274 798aa Protein coding CCDS7174 Q5T3E6 - TSL:5, GENCODE basic, APPRIS ALT1,
ITGB1-231 ENST00000678701.1 4090 798aa Protein coding CCDS7174 - - GENCODE basic, APPRIS ALT1,
ITGB1-233 ENST00000678943.1 4001 798aa Protein coding CCDS7174 - - GENCODE basic, APPRIS ALT1,
ITGB1-204 ENST00000417122.7 3982 798aa Protein coding CCDS7174 Q5T3E5 - TSL:4, GENCODE basic, APPRIS ALT1,
ITGB1-227 ENST00000677999.1 3918 798aa Protein coding CCDS7174 - - GENCODE basic, APPRIS ALT1,
ITGB1-234 ENST00000678952.1 3815 798aa Protein coding CCDS7174 - - GENCODE basic, APPRIS ALT1,
ITGB1-202 ENST00000396033.6 3784 798aa Protein coding CCDS7174 P05556-1 - TSL:5, GENCODE basic, APPRIS P3,
ITGB1-201 ENST00000302278.8 3735 798aa Protein coding CCDS7174 P05556-1 NM_002211.4 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
ITGB1-226 ENST00000677363.1 4809 809aa Protein coding - - - GENCODE basic,
ITGB1-220 ENST00000676460.1 4489 789aa Protein coding - - - GENCODE basic,
ITGB1-219 ENST00000609742.3 4170 793aa Protein coding - - - TSL:NA, GENCODE basic,
ITGB1-230 ENST00000678591.1 4097 794aa Protein coding - - - GENCODE basic,
ITGB1-210 ENST00000474568.6 4021 809aa Protein coding - C9JPK5 - TSL:5, GENCODE basic,
ITGB1-225 ENST00000677310.1 4010 825aa Protein coding - - - GENCODE basic,
ITGB1-224 ENST00000676964.1 3955 741aa Protein coding - - - GENCODE basic,
ITGB1-236 ENST00000679187.1 3839 741aa Protein coding - - - GENCODE basic,
ITGB1-232 ENST00000678766.1 3780 825aa Protein coding - - - GENCODE basic,
ITGB1-222 ENST00000676659.1 3779 819aa Protein coding - - - GENCODE basic,
ITGB1-205 ENST00000423113.5 3729 801aa Protein coding - P05556-5 - TSL:5, GENCODE basic, APPRIS ALT1,
ITGB1-215 ENST00000488494.6 3726 801aa Protein coding - E7EUI6 - TSL:4, GENCODE basic, APPRIS ALT1,
ITGB1-214 ENST00000488427.2 3707 741aa Protein coding - H7C4K3 - TSL:1, GENCODE basic,
ITGB1-209 ENST00000472147.1 668 14aa Protein coding - C9JNE0 - CDS 3' incomplete, TSL:3,
ITGB1-212 ENST00000480226.5 585 163aa Protein coding - E7EQW5 - CDS 3' incomplete, TSL:4,
ITGB1-218 ENST00000534049.5 562 143aa Protein coding - E9PLR6 - CDS 3' incomplete, TSL:4,
ITGB1-217 ENST00000528877.5 546 104aa Protein coding - E9PQJ2 - CDS 3' incomplete, TSL:4,
ITGB1-203 ENST00000414670.2 500 49aa Protein coding - Q5T3E4 - CDS 3' incomplete, TSL:3,
ITGB1-235 ENST00000678989.1 4307 798aa Nonsense mediated decay CCDS7174 - - -
ITGB1-223 ENST00000676895.1 3875 380aa Nonsense mediated decay - - - -
ITGB1-228 ENST00000678120.1 3755 379aa Nonsense mediated decay - - - -
ITGB1-229 ENST00000678479.1 3741 65aa Nonsense mediated decay - - - -
ITGB1-216 ENST00000494395.1 562 108aa Nonsense mediated decay - H7C4N8 - CDS 5' incomplete, TSL:3,
ITGB1-213 ENST00000484088.5 580 No protein Processed transcript - - - TSL:4,
ITGB1-221 ENST00000676623.1 5614 No protein Retained intron - - - -
ITGB1-211 ENST00000475184.6 1927 No protein Retained intron - - - TSL:4,
ITGB1-207 ENST00000439974.3 580 No protein Retained intron - - - TSL:5,
ITGB1-208 ENST00000464001.1 316 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ITGB1
This KO Strategy loading
Red Cotton™ Notes Gene ITGB1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×