CRISPR-U™ Gene Knockout Cell Line
Strategy
ITGB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ITGB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ITGB1 |
| Gene id | 3688 |
| Organism | Homo sapiens |
| Official full symbol | integrin subunit beta 1 |
| Gene type | protein-coding |
| Also known as | CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB |
| Summary | Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 26 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ITGB1-206 | ENST00000437302.6 | 4274 | 798aa | Protein coding | CCDS7174 | Q5T3E6 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| ITGB1-231 | ENST00000678701.1 | 4090 | 798aa | Protein coding | CCDS7174 | - | - | GENCODE basic, APPRIS ALT1, |
| ITGB1-233 | ENST00000678943.1 | 4001 | 798aa | Protein coding | CCDS7174 | - | - | GENCODE basic, APPRIS ALT1, |
| ITGB1-204 | ENST00000417122.7 | 3982 | 798aa | Protein coding | CCDS7174 | Q5T3E5 | - | TSL:4, GENCODE basic, APPRIS ALT1, |
| ITGB1-227 | ENST00000677999.1 | 3918 | 798aa | Protein coding | CCDS7174 | - | - | GENCODE basic, APPRIS ALT1, |
| ITGB1-234 | ENST00000678952.1 | 3815 | 798aa | Protein coding | CCDS7174 | - | - | GENCODE basic, APPRIS ALT1, |
| ITGB1-202 | ENST00000396033.6 | 3784 | 798aa | Protein coding | CCDS7174 | P05556-1 | - | TSL:5, GENCODE basic, APPRIS P3, |
| ITGB1-201 | ENST00000302278.8 | 3735 | 798aa | Protein coding | CCDS7174 | P05556-1 | NM_002211.4 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| ITGB1-226 | ENST00000677363.1 | 4809 | 809aa | Protein coding | - | - | - | GENCODE basic, |
| ITGB1-220 | ENST00000676460.1 | 4489 | 789aa | Protein coding | - | - | - | GENCODE basic, |
| ITGB1-219 | ENST00000609742.3 | 4170 | 793aa | Protein coding | - | - | - | TSL:NA, GENCODE basic, |
| ITGB1-230 | ENST00000678591.1 | 4097 | 794aa | Protein coding | - | - | - | GENCODE basic, |
| ITGB1-210 | ENST00000474568.6 | 4021 | 809aa | Protein coding | - | C9JPK5 | - | TSL:5, GENCODE basic, |
| ITGB1-225 | ENST00000677310.1 | 4010 | 825aa | Protein coding | - | - | - | GENCODE basic, |
| ITGB1-224 | ENST00000676964.1 | 3955 | 741aa | Protein coding | - | - | - | GENCODE basic, |
| ITGB1-236 | ENST00000679187.1 | 3839 | 741aa | Protein coding | - | - | - | GENCODE basic, |
| ITGB1-232 | ENST00000678766.1 | 3780 | 825aa | Protein coding | - | - | - | GENCODE basic, |
| ITGB1-222 | ENST00000676659.1 | 3779 | 819aa | Protein coding | - | - | - | GENCODE basic, |
| ITGB1-205 | ENST00000423113.5 | 3729 | 801aa | Protein coding | - | P05556-5 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| ITGB1-215 | ENST00000488494.6 | 3726 | 801aa | Protein coding | - | E7EUI6 | - | TSL:4, GENCODE basic, APPRIS ALT1, |
| ITGB1-214 | ENST00000488427.2 | 3707 | 741aa | Protein coding | - | H7C4K3 | - | TSL:1, GENCODE basic, |
| ITGB1-209 | ENST00000472147.1 | 668 | 14aa | Protein coding | - | C9JNE0 | - | CDS 3' incomplete, TSL:3, |
| ITGB1-212 | ENST00000480226.5 | 585 | 163aa | Protein coding | - | E7EQW5 | - | CDS 3' incomplete, TSL:4, |
| ITGB1-218 | ENST00000534049.5 | 562 | 143aa | Protein coding | - | E9PLR6 | - | CDS 3' incomplete, TSL:4, |
| ITGB1-217 | ENST00000528877.5 | 546 | 104aa | Protein coding | - | E9PQJ2 | - | CDS 3' incomplete, TSL:4, |
| ITGB1-203 | ENST00000414670.2 | 500 | 49aa | Protein coding | - | Q5T3E4 | - | CDS 3' incomplete, TSL:3, |
| ITGB1-235 | ENST00000678989.1 | 4307 | 798aa | Nonsense mediated decay | CCDS7174 | - | - | - |
| ITGB1-223 | ENST00000676895.1 | 3875 | 380aa | Nonsense mediated decay | - | - | - | - |
| ITGB1-228 | ENST00000678120.1 | 3755 | 379aa | Nonsense mediated decay | - | - | - | - |
| ITGB1-229 | ENST00000678479.1 | 3741 | 65aa | Nonsense mediated decay | - | - | - | - |
| ITGB1-216 | ENST00000494395.1 | 562 | 108aa | Nonsense mediated decay | - | H7C4N8 | - | CDS 5' incomplete, TSL:3, |
| ITGB1-213 | ENST00000484088.5 | 580 | No protein | Processed transcript | - | - | - | TSL:4, |
| ITGB1-221 | ENST00000676623.1 | 5614 | No protein | Retained intron | - | - | - | - |
| ITGB1-211 | ENST00000475184.6 | 1927 | No protein | Retained intron | - | - | - | TSL:4, |
| ITGB1-207 | ENST00000439974.3 | 580 | No protein | Retained intron | - | - | - | TSL:5, |
| ITGB1-208 | ENST00000464001.1 | 316 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ITGB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ITGB1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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