CRISPR-U™ Gene Knockout Cell Line Strategy

MDH2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MDH2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MDH2
Gene id 4191
Organism Homo sapiens
Official full symbol malate dehydrogenase 2
Gene type protein-coding
Also known as EIEE51, M-MDH, MDH, MGC:3559, MOR1
Summary Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 7
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MDH2-201 ENST00000315758.10 2170 338aa Protein coding CCDS5581 P40926-1 NM_005918.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MDH2-204 ENST00000443006.5 2020 231aa Protein coding CCDS75622 G3XAL0 - TSL:2, GENCODE basic,
MDH2-203 ENST00000432020.2 1548 296aa Protein coding CCDS64691 P40926-2 - TSL:2, GENCODE basic,
MDH2-205 ENST00000461263.2 648 28aa Protein coding - U3KQ63 - TSL:2, GENCODE basic,
MDH2-207 ENST00000490105.1 569 No protein Processed transcript - - - TSL:3,
MDH2-202 ENST00000424167.2 681 No protein Retained intron - - - TSL:2,
MDH2-206 ENST00000461665.5 551 No protein Retained intron - - - TSL:2,
MDH2-208 ENST00000492663.1 546 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MDH2
This KO Strategy loading
Red Cotton™ Notes Gene MDH2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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