CRISPR-U™ Gene Knockout Cell Line
Strategy
MDH2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MDH2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MDH2 |
| Gene id | 4191 |
| Organism | Homo sapiens |
| Official full symbol | malate dehydrogenase 2 |
| Gene type | protein-coding |
| Also known as | EIEE51, M-MDH, MDH, MGC:3559, MOR1 |
| Summary | Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MDH2-201 | ENST00000315758.10 | 2170 | 338aa | Protein coding | CCDS5581 | P40926-1 | NM_005918.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MDH2-204 | ENST00000443006.5 | 2020 | 231aa | Protein coding | CCDS75622 | G3XAL0 | - | TSL:2, GENCODE basic, |
| MDH2-203 | ENST00000432020.2 | 1548 | 296aa | Protein coding | CCDS64691 | P40926-2 | - | TSL:2, GENCODE basic, |
| MDH2-205 | ENST00000461263.2 | 648 | 28aa | Protein coding | - | U3KQ63 | - | TSL:2, GENCODE basic, |
| MDH2-207 | ENST00000490105.1 | 569 | No protein | Processed transcript | - | - | - | TSL:3, |
| MDH2-202 | ENST00000424167.2 | 681 | No protein | Retained intron | - | - | - | TSL:2, |
| MDH2-206 | ENST00000461665.5 | 551 | No protein | Retained intron | - | - | - | TSL:2, |
| MDH2-208 | ENST00000492663.1 | 546 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MDH2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MDH2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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