CRISPR-U™ Gene Knockout Cell Line Strategy

LMNB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LMNB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LMNB1
Gene id 4001
Organism Homo sapiens
Official full symbol lamin B1
Gene type protein-coding
Also known as ADLD, LMN, LMN2, LMNB
Summary This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 5
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LMNB1-201 ENST00000261366.10 2890 586aa Protein coding CCDS4140 P20700 NM_005573.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LMNB1-202 ENST00000395354.1 1572 387aa Protein coding - E9PBF6 - TSL:1, GENCODE basic,
LMNB1-206 ENST00000492190.5 738 166aa Protein coding - A0A0D9SFY5 - CDS 3' incomplete, TSL:3,
LMNB1-203 ENST00000460265.5 3475 329aa Nonsense mediated decay - A0A0D9SFE5 - TSL:1,
LMNB1-208 ENST00000504788.5 1801 No protein Processed transcript - - - TSL:2,
LMNB1-205 ENST00000472034.5 730 No protein Processed transcript - - - TSL:3,
LMNB1-204 ENST00000463908.2 557 No protein Processed transcript - - - TSL:4,
LMNB1-207 ENST00000494185.1 579 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LMNB1
This KO Strategy loading
Red Cotton™ Notes Gene LMNB1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×