CRISPR-U™ Gene Knockout Cell Line
Strategy
LMNB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LMNB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LMNB1 |
| Gene id | 4001 |
| Organism | Homo sapiens |
| Official full symbol | lamin B1 |
| Gene type | protein-coding |
| Also known as | ADLD, LMN, LMN2, LMNB |
| Summary | This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LMNB1-201 | ENST00000261366.10 | 2890 | 586aa | Protein coding | CCDS4140 | P20700 | NM_005573.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LMNB1-202 | ENST00000395354.1 | 1572 | 387aa | Protein coding | - | E9PBF6 | - | TSL:1, GENCODE basic, |
| LMNB1-206 | ENST00000492190.5 | 738 | 166aa | Protein coding | - | A0A0D9SFY5 | - | CDS 3' incomplete, TSL:3, |
| LMNB1-203 | ENST00000460265.5 | 3475 | 329aa | Nonsense mediated decay | - | A0A0D9SFE5 | - | TSL:1, |
| LMNB1-208 | ENST00000504788.5 | 1801 | No protein | Processed transcript | - | - | - | TSL:2, |
| LMNB1-205 | ENST00000472034.5 | 730 | No protein | Processed transcript | - | - | - | TSL:3, |
| LMNB1-204 | ENST00000463908.2 | 557 | No protein | Processed transcript | - | - | - | TSL:4, |
| LMNB1-207 | ENST00000494185.1 | 579 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LMNB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LMNB1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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