CRISPR-U™ Gene Knockout Cell Line Strategy

BAX Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BAX Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BAX
Gene id 581
Organism Homo sapiens
Official full symbol BCL2 associated X, apoptosis regulator
Gene type protein-coding
Also known as BCL2L4
Summary The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BAX-201 ENST00000293288.12 1358 218aa Protein coding CCDS12744 Q07812-2 - TSL:1, GENCODE basic,
BAX-202 ENST00000345358.12 795 192aa Protein coding CCDS12742 Q07812-1 NM_138761.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BAX-206 ENST00000415969.6 540 179aa Protein coding CCDS12745 Q07812-8 - TSL:1, GENCODE basic,
BAX-213 ENST00000539787.2 458 140aa Protein coding CCDS77327 I6LPK7 - TSL:1, GENCODE basic,
BAX-203 ENST00000354470.7 433 143aa Protein coding CCDS12743 Q07812-4 - TSL:1, GENCODE basic,
BAX-205 ENST00000391871.4 687 175aa Protein coding - K4JQN1 - TSL:5, GENCODE basic,
BAX-209 ENST00000506183.5 383 126aa Protein coding - H0YA56 - CDS 5' incomplete, TSL:1,
BAX-204 ENST00000356483.8 677 164aa Nonsense mediated decay - Q07812-5 - TSL:1,
BAX-212 ENST00000515540.5 300 41aa Nonsense mediated decay - Q07812-3 - TSL:3,
BAX-207 ENST00000502487.5 1346 No protein Retained intron - - - TSL:1,
BAX-210 ENST00000513217.1 869 No protein Retained intron - - - TSL:2,
BAX-211 ENST00000513545.5 775 No protein Retained intron - - - TSL:3,
BAX-208 ENST00000503726.2 488 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BAX
This KO Strategy loading
Red Cotton™ Notes Gene BAX had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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