CRISPR-U™ Gene Knockout Cell Line
Strategy
BAX Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BAX Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BAX |
| Gene id | 581 |
| Organism | Homo sapiens |
| Official full symbol | BCL2 associated X, apoptosis regulator |
| Gene type | protein-coding |
| Also known as | BCL2L4 |
| Summary | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BAX-201 | ENST00000293288.12 | 1358 | 218aa | Protein coding | CCDS12744 | Q07812-2 | - | TSL:1, GENCODE basic, |
| BAX-202 | ENST00000345358.12 | 795 | 192aa | Protein coding | CCDS12742 | Q07812-1 | NM_138761.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BAX-206 | ENST00000415969.6 | 540 | 179aa | Protein coding | CCDS12745 | Q07812-8 | - | TSL:1, GENCODE basic, |
| BAX-213 | ENST00000539787.2 | 458 | 140aa | Protein coding | CCDS77327 | I6LPK7 | - | TSL:1, GENCODE basic, |
| BAX-203 | ENST00000354470.7 | 433 | 143aa | Protein coding | CCDS12743 | Q07812-4 | - | TSL:1, GENCODE basic, |
| BAX-205 | ENST00000391871.4 | 687 | 175aa | Protein coding | - | K4JQN1 | - | TSL:5, GENCODE basic, |
| BAX-209 | ENST00000506183.5 | 383 | 126aa | Protein coding | - | H0YA56 | - | CDS 5' incomplete, TSL:1, |
| BAX-204 | ENST00000356483.8 | 677 | 164aa | Nonsense mediated decay | - | Q07812-5 | - | TSL:1, |
| BAX-212 | ENST00000515540.5 | 300 | 41aa | Nonsense mediated decay | - | Q07812-3 | - | TSL:3, |
| BAX-207 | ENST00000502487.5 | 1346 | No protein | Retained intron | - | - | - | TSL:1, |
| BAX-210 | ENST00000513217.1 | 869 | No protein | Retained intron | - | - | - | TSL:2, |
| BAX-211 | ENST00000513545.5 | 775 | No protein | Retained intron | - | - | - | TSL:3, |
| BAX-208 | ENST00000503726.2 | 488 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BAX |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BAX had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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