CRISPR-U™ Gene Knockout Cell Line
Strategy
MVP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MVP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MVP |
| Gene id | 9961 |
| Organism | Homo sapiens |
| Official full symbol | major vault protein |
| Gene type | protein-coding |
| Also known as | LRP, VAULT1 |
| Summary | This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MVP-202 | ENST00000395353.5 | 2925 | 893aa | Protein coding | CCDS10656 | Q14764 X5D2M8 | - | TSL:5, GENCODE basic, APPRIS P1, |
| MVP-201 | ENST00000357402.10 | 2798 | 893aa | Protein coding | CCDS10656 | Q14764 X5D2M8 | NM_005115.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MVP-207 | ENST00000563915.5 | 854 | 220aa | Protein coding | - | H3BQK6 | - | CDS 3' incomplete, TSL:3, |
| MVP-218 | ENST00000570234.5 | 658 | 148aa | Protein coding | - | H3BRL2 | - | CDS 3' incomplete, TSL:4, |
| MVP-206 | ENST00000563558.5 | 548 | 144aa | Protein coding | - | H3BNF6 | - | CDS 3' incomplete, TSL:4, |
| MVP-210 | ENST00000566066.5 | 548 | 94aa | Protein coding | - | H3BP76 | - | CDS 3' incomplete, TSL:4, |
| MVP-211 | ENST00000566252.1 | 408 | 107aa | Protein coding | - | H3BUK7 | - | CDS 3' incomplete, TSL:3, |
| MVP-208 | ENST00000565164.1 | 211 | 42aa | Protein coding | - | H3BNF2 | - | CDS 3' incomplete, TSL:5, |
| MVP-219 | ENST00000629059.1 | 120 | 39aa | Protein coding | - | H3BPZ2 | - | TSL:5, GENCODE basic, |
| MVP-213 | ENST00000566859.5 | 589 | 81aa | Nonsense mediated decay | - | H3BQE7 | - | TSL:5, |
| MVP-205 | ENST00000563123.1 | 575 | 61aa | Nonsense mediated decay | - | I3L155 | - | CDS 5' incomplete, TSL:4, |
| MVP-216 | ENST00000569887.1 | 549 | 39aa | Nonsense mediated decay | - | H3BPZ2 | - | TSL:5, |
| MVP-203 | ENST00000562463.5 | 525 | 57aa | Nonsense mediated decay | - | H3BUP3 | - | TSL:4, |
| MVP-212 | ENST00000566554.1 | 811 | No protein | Processed transcript | - | - | - | TSL:4, |
| MVP-217 | ENST00000570061.1 | 674 | No protein | Processed transcript | - | - | - | TSL:4, |
| MVP-209 | ENST00000565830.5 | 557 | No protein | Processed transcript | - | - | - | TSL:4, |
| MVP-215 | ENST00000569612.1 | 2091 | No protein | Retained intron | - | - | - | TSL:1, |
| MVP-214 | ENST00000568068.1 | 561 | No protein | Retained intron | - | - | - | TSL:2, |
| MVP-204 | ENST00000563096.1 | 546 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Levelloading
| Target Gene | MVP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MVP had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Work flow
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