CRISPR-U™ Gene Knockout Cell Line
Strategy
IL6ST Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IL6ST Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IL6ST |
| Gene id | 3572 |
| Organism | Homo sapiens |
| Official full symbol | interleukin 6 signal transducer |
| Gene type | protein-coding |
| Also known as | CD130, CDW130, GP130, HIES4, IL-6RB, sGP130 |
| Summary | The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IL6ST-206 | ENST00000381298.7 | 9027 | 918aa | Protein coding | CCDS3971 | P40189-1 | NM_002184.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IL6ST-201 | ENST00000336909.9 | 8776 | 918aa | Protein coding | CCDS3971 | P40189-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| IL6ST-203 | ENST00000381287.8 | 8667 | 329aa | Protein coding | CCDS47209 | P40189-2 | - | TSL:5, GENCODE basic, |
| IL6ST-209 | ENST00000502326.7 | 3007 | 918aa | Protein coding | CCDS3971 | P40189-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| IL6ST-205 | ENST00000381294.7 | 2574 | 857aa | Protein coding | CCDS54856 | P40189-3 | - | TSL:1, GENCODE basic, |
| IL6ST-212 | ENST00000522633.2 | 1878 | 329aa | Protein coding | CCDS47209 | P40189-2 | - | TSL:1, GENCODE basic, |
| IL6ST-204 | ENST00000381293.6 | 612 | 203aa | Protein coding | - | Q5FC05 | - | TSL:1, GENCODE basic, |
| IL6ST-202 | ENST00000381286.7 | 201 | 66aa | Protein coding | - | Q5FC02 | - | TSL:1, GENCODE basic, |
| IL6ST-207 | ENST00000396816.1 | 168 | 55aa | Protein coding | - | Q5FC07 | - | TSL:1, GENCODE basic, |
| IL6ST-216 | ENST00000651614.1 | 8412 | 642aa | Nonsense mediated decay | - | A0A494BZU0 | - | - |
| IL6ST-210 | ENST00000503773.6 | 3031 | 333aa | Nonsense mediated decay | - | D6RH03 | - | TSL:5, |
| IL6ST-214 | ENST00000577363.1 | 282 | No protein | Processed transcript | - | - | - | TSL:5, |
| IL6ST-208 | ENST00000423954.3 | 153 | No protein | Processed transcript | - | - | - | TSL:1, |
| IL6ST-213 | ENST00000523039.5 | 481 | No protein | Retained intron | - | - | - | TSL:2, |
| IL6ST-215 | ENST00000583149.1 | 479 | No protein | Retained intron | - | - | - | TSL:2, |
| IL6ST-211 | ENST00000506241.1 | 418 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IL6ST |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IL6ST had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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