CRISPR-U™ Gene Knockout Cell Line Strategy

IL6ST Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IL6ST Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IL6ST
Gene id 3572
Organism Homo sapiens
Official full symbol interleukin 6 signal transducer
Gene type protein-coding
Also known as CD130, CDW130, GP130, HIES4, IL-6RB, sGP130
Summary The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17.
Genomic regions Chromosome 5
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IL6ST-206 ENST00000381298.7 9027 918aa Protein coding CCDS3971 P40189-1 NM_002184.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IL6ST-201 ENST00000336909.9 8776 918aa Protein coding CCDS3971 P40189-1 - TSL:2, GENCODE basic, APPRIS P1,
IL6ST-203 ENST00000381287.8 8667 329aa Protein coding CCDS47209 P40189-2 - TSL:5, GENCODE basic,
IL6ST-209 ENST00000502326.7 3007 918aa Protein coding CCDS3971 P40189-1 - TSL:5, GENCODE basic, APPRIS P1,
IL6ST-205 ENST00000381294.7 2574 857aa Protein coding CCDS54856 P40189-3 - TSL:1, GENCODE basic,
IL6ST-212 ENST00000522633.2 1878 329aa Protein coding CCDS47209 P40189-2 - TSL:1, GENCODE basic,
IL6ST-204 ENST00000381293.6 612 203aa Protein coding - Q5FC05 - TSL:1, GENCODE basic,
IL6ST-202 ENST00000381286.7 201 66aa Protein coding - Q5FC02 - TSL:1, GENCODE basic,
IL6ST-207 ENST00000396816.1 168 55aa Protein coding - Q5FC07 - TSL:1, GENCODE basic,
IL6ST-216 ENST00000651614.1 8412 642aa Nonsense mediated decay - A0A494BZU0 - -
IL6ST-210 ENST00000503773.6 3031 333aa Nonsense mediated decay - D6RH03 - TSL:5,
IL6ST-214 ENST00000577363.1 282 No protein Processed transcript - - - TSL:5,
IL6ST-208 ENST00000423954.3 153 No protein Processed transcript - - - TSL:1,
IL6ST-213 ENST00000523039.5 481 No protein Retained intron - - - TSL:2,
IL6ST-215 ENST00000583149.1 479 No protein Retained intron - - - TSL:2,
IL6ST-211 ENST00000506241.1 418 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IL6ST
This KO Strategy loading
Red Cotton™ Notes Gene IL6ST had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×