CRISPR-U™ Gene Knockout Cell Line
Strategy
BAK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BAK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BAK1 |
| Gene id | 578 |
| Organism | Homo sapiens |
| Official full symbol | BCL2 antagonist/killer 1 |
| Gene type | protein-coding |
| Also known as | BAK, BAK-LIKE, BCL2L7, CDN1 |
| Summary | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BAK1-202 | ENST00000374467.4 | 2170 | 211aa | Protein coding | CCDS4781 | Q16611-1 | NM_001188.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BAK1-203 | ENST00000442998.6 | 2212 | 153aa | Protein coding | - | Q16611-2 | - | TSL:1, GENCODE basic, |
| BAK1-201 | ENST00000360661.9 | 2132 | 191aa | Protein coding | - | A0A0A0MRG8 | - | TSL:5, GENCODE basic, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BAK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BAK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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