CRISPR-U™ Gene Knockout Cell Line
Strategy
CDH2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDH2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDH2 |
| Gene id | 1000 |
| Organism | Homo sapiens |
| Official full symbol | cadherin 2 |
| Gene type | protein-coding |
| Also known as | ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD |
| Summary | This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. |
| Genomic regions | Chromosome 18 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDH2-201 | ENST00000269141.8 | 4016 | 906aa | Protein coding | CCDS11891 | P19022-1 | NM_001792.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CDH2-202 | ENST00000399380.7 | 3737 | 875aa | Protein coding | CCDS77172 | P19022-2 | - | TSL:2, GENCODE basic, |
| CDH2-211 | ENST00000676445.1 | 3243 | 821aa | Protein coding | - | - | - | GENCODE basic, |
| CDH2-205 | ENST00000430882.6 | 3143 | 821aa | Protein coding | - | C9J126 | - | TSL:4, |
| CDH2-203 | ENST00000413878.2 | 2574 | 691aa | Protein coding | - | C9JMH2 | - | CDS 3' incomplete, TSL:4, |
| CDH2-207 | ENST00000675173.1 | 1397 | 394aa | Protein coding | - | - | - | CDS 5' incomplete, |
| CDH2-204 | ENST00000418492.5 | 740 | 191aa | Protein coding | - | C9J8J8 | - | CDS 3' incomplete, TSL:5, |
| CDH2-209 | ENST00000675708.1 | 3860 | 733aa | Nonsense mediated decay | - | - | - | - |
| CDH2-208 | ENST00000675688.1 | 899 | 161aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| CDH2-206 | ENST00000674998.1 | 3627 | No protein | Retained intron | - | - | - | - |
| CDH2-210 | ENST00000676041.1 | 2947 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDH2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDH2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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