CRISPR-U™ Gene Knockout Cell Line Strategy

CDH2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDH2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDH2
Gene id 1000
Organism Homo sapiens
Official full symbol cadherin 2
Gene type protein-coding
Also known as ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD
Summary This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Genomic regions Chromosome 18
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDH2-201 ENST00000269141.8 4016 906aa Protein coding CCDS11891 P19022-1 NM_001792.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDH2-202 ENST00000399380.7 3737 875aa Protein coding CCDS77172 P19022-2 - TSL:2, GENCODE basic,
CDH2-211 ENST00000676445.1 3243 821aa Protein coding - - - GENCODE basic,
CDH2-205 ENST00000430882.6 3143 821aa Protein coding - C9J126 - TSL:4,
CDH2-203 ENST00000413878.2 2574 691aa Protein coding - C9JMH2 - CDS 3' incomplete, TSL:4,
CDH2-207 ENST00000675173.1 1397 394aa Protein coding - - - CDS 5' incomplete,
CDH2-204 ENST00000418492.5 740 191aa Protein coding - C9J8J8 - CDS 3' incomplete, TSL:5,
CDH2-209 ENST00000675708.1 3860 733aa Nonsense mediated decay - - - -
CDH2-208 ENST00000675688.1 899 161aa Nonsense mediated decay - - - CDS 5' incomplete,
CDH2-206 ENST00000674998.1 3627 No protein Retained intron - - - -
CDH2-210 ENST00000676041.1 2947 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDH2
This KO Strategy loading
Red Cotton™ Notes Gene CDH2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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