CRISPR-U™ Gene Knockout Cell Line Strategy

CHEK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CHEK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CHEK2
Gene id 11200
Organism Homo sapiens
Official full symbol checkpoint kinase 2
Gene type protein-coding
Also known as CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
Summary In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 22
Strategy Summary
This gene has 15 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CHEK2-202 ENST00000382580.6 1971 586aa Protein coding CCDS33629 O96017-9 - TSL:1, GENCODE basic,
CHEK2-207 ENST00000405598.5 1959 543aa Protein coding CCDS13843 O96017-1 - TSL:5, GENCODE basic, APPRIS P1,
CHEK2-225 ENST00000650281.1 1923 543aa Protein coding CCDS13843 O96017-1 - GENCODE basic, APPRIS P1,
CHEK2-206 ENST00000404276.6 1844 543aa Protein coding CCDS13843 O96017-1 NM_007194.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CHEK2-201 ENST00000348295.7 1771 514aa Protein coding CCDS13844 O96017-12 - TSL:5, GENCODE basic,
CHEK2-204 ENST00000402731.6 1591 476aa Protein coding - O96017-12 - TSL:1, GENCODE basic,
CHEK2-210 ENST00000425190.7 1510 322aa Protein coding - B7ZBF2 - TSL:5, GENCODE basic,
CHEK2-205 ENST00000403642.5 1359 452aa Protein coding - O96017-4 - TSL:1, GENCODE basic,
CHEK2-223 ENST00000649563.1 1224 322aa Protein coding - O96017-13 - GENCODE basic,
CHEK2-219 ENST00000464581.6 1138 323aa Protein coding - A0A087X102 - TSL:2, GENCODE basic,
CHEK2-213 ENST00000434810.5 984 276aa Protein coding - H0Y820 - CDS 5' incomplete, TSL:3,
CHEK2-214 ENST00000439200.5 906 295aa Protein coding - B7ZBF7 - CDS 3' incomplete, TSL:5,
CHEK2-203 ENST00000398017.3 713 162aa Protein coding - B7ZBF8 - CDS 3' incomplete, TSL:5,
CHEK2-218 ENST00000456369.5 588 144aa Protein coding - H0Y4V6 - CDS 5' incomplete, TSL:3,
CHEK2-224 ENST00000650233.1 573 176aa Protein coding - A0A3B3ITA7 - CDS 3' incomplete,
CHEK2-208 ENST00000416671.5 2560 207aa Nonsense mediated decay - C9JFD7 - TSL:1,
CHEK2-212 ENST00000433728.5 1580 289aa Nonsense mediated decay - O96017-8 - TSL:2,
CHEK2-209 ENST00000417588.5 1551 203aa Nonsense mediated decay - O96017-5 - TSL:2,
CHEK2-216 ENST00000448511.5 1532 165aa Nonsense mediated decay - O96017-6 - TSL:2,
CHEK2-211 ENST00000433028.6 1349 161aa Nonsense mediated decay - F8WCV2 - TSL:5,
CHEK2-217 ENST00000454252.1 722 45aa Nonsense mediated decay - H7BZ30 - CDS 5' incomplete, TSL:3,
CHEK2-215 ENST00000439346.5 553 57aa Nonsense mediated decay - H7C0V7 - CDS 5' incomplete, TSL:4,
CHEK2-222 ENST00000648295.1 1337 No protein Processed transcript - - - -
CHEK2-221 ENST00000491919.5 609 No protein Retained intron - - - TSL:3,
CHEK2-220 ENST00000472807.1 514 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CHEK2
This KO Strategy loading
Red Cotton™ Notes Gene CHEK2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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