CRISPR-U™ Gene Knockout Cell Line
Strategy
ATF3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATF3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATF3 |
| Gene id | 467 |
| Organism | Homo sapiens |
| Official full symbol | activating transcription factor 3 |
| Gene type | protein-coding |
| Summary | This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATF3-211 | ENST00000613954.4 | 2031 | 124aa | Protein coding | CCDS58059 | P18847-5 | - | TSL:5, GENCODE basic, |
| ATF3-202 | ENST00000341491.9 | 1940 | 181aa | Protein coding | CCDS1506 | P18847-1 | NM_001674.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ATF3-206 | ENST00000366987.6 | 1917 | 181aa | Protein coding | CCDS1506 | P18847-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| ATF3-204 | ENST00000366983.5 | 858 | 135aa | Protein coding | CCDS41464 | P18847-3 | - | TSL:1, GENCODE basic, |
| ATF3-210 | ENST00000613104.1 | 581 | 124aa | Protein coding | CCDS58059 | P18847-5 | - | TSL:1, GENCODE basic, |
| ATF3-201 | ENST00000336937.8 | 482 | 106aa | Protein coding | CCDS55688 | P18847-4 | - | TSL:1, GENCODE basic, |
| ATF3-203 | ENST00000366981.8 | 680 | 175aa | Protein coding | - | Q5VTZ4 | - | CDS 3' incomplete, TSL:1, |
| ATF3-205 | ENST00000366985.5 | 516 | 153aa | Protein coding | - | A0A0A0MRJ2 | - | CDS 5' incomplete, TSL:5, |
| ATF3-207 | ENST00000464547.5 | 767 | 135aa | Nonsense mediated decay | CCDS41464 | P18847-3 | - | TSL:1, |
| ATF3-209 | ENST00000492118.2 | 2103 | No protein | Processed transcript | - | - | - | TSL:2, |
| ATF3-208 | ENST00000465155.5 | 1626 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATF3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATF3 had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: