CRISPR-U™ Gene Knockout Cell Line
Strategy
ADAR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ADAR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ADAR |
| Gene id | 103 |
| Organism | Homo sapiens |
| Official full symbol | adenosine deaminase RNA specific |
| Gene type | protein-coding |
| Also known as | ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136 |
| Summary | This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 15 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ADAR-211 | ENST00000648231.2 | 6971 | 931aa | Protein coding | CCDS30879 | P55265-5 | - | GENCODE basic, |
| ADAR-225 | ENST00000680270.1 | 6664 | 931aa | Protein coding | CCDS30879 | - | - | GENCODE basic, |
| ADAR-216 | ENST00000649022.2 | 6631 | 931aa | Protein coding | CCDS30879 | A0A3B3IRQ9 | - | GENCODE basic, |
| ADAR-202 | ENST00000368474.9 | 6610 | 1226aa | Protein coding | CCDS1071 | P55265-1 | NM_001111.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ADAR-212 | ENST00000648311.1 | 6539 | 931aa | Protein coding | CCDS30879 | P55265-5 | - | GENCODE basic, |
| ADAR-201 | ENST00000368471.8 | 6519 | 931aa | Protein coding | CCDS30879 | P55265-5 | - | TSL:1, GENCODE basic, |
| ADAR-228 | ENST00000681056.1 | 6450 | 931aa | Protein coding | CCDS30879 | - | - | GENCODE basic, |
| ADAR-219 | ENST00000649724.1 | 6425 | 931aa | Protein coding | CCDS30879 | P55265-5 | - | GENCODE basic, |
| ADAR-220 | ENST00000649749.1 | 6412 | 931aa | Protein coding | CCDS30879 | P55265-5 | - | GENCODE basic, |
| ADAR-217 | ENST00000649042.1 | 6263 | 931aa | Protein coding | CCDS30879 | P55265-5 | - | GENCODE basic, |
| ADAR-231 | ENST00000681683.1 | 4080 | 931aa | Protein coding | CCDS30879 | - | - | GENCODE basic, |
| ADAR-224 | ENST00000679899.1 | 6788 | 912aa | Protein coding | - | - | - | GENCODE basic, |
| ADAR-226 | ENST00000680305.1 | 6427 | 1165aa | Protein coding | - | - | - | GENCODE basic, |
| ADAR-208 | ENST00000529168.2 | 6343 | 1200aa | Protein coding | - | P55265-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| ADAR-214 | ENST00000648871.1 | 1619 | 350aa | Protein coding | - | A0A3B3ITG9 | - | CDS 3' incomplete, |
| ADAR-233 | ENST00000681901.1 | 6962 | 87aa | Nonsense mediated decay | - | A2IBT1 | - | - |
| ADAR-218 | ENST00000649408.2 | 6915 | 1046aa | Nonsense mediated decay | - | A0A3B3ISU1 | - | - |
| ADAR-213 | ENST00000648714.2 | 6832 | 788aa | Nonsense mediated decay | - | A0A3B3ISX1 | - | - |
| ADAR-229 | ENST00000681235.1 | 6753 | 87aa | Nonsense mediated decay | - | A2IBT1 | - | - |
| ADAR-221 | ENST00000679375.1 | 6386 | 263aa | Nonsense mediated decay | - | - | - | - |
| ADAR-204 | ENST00000471068.2 | 583 | No protein | Processed transcript | - | - | - | TSL:4, |
| ADAR-206 | ENST00000494866.1 | 471 | No protein | Processed transcript | - | - | - | TSL:2, |
| ADAR-207 | ENST00000526905.2 | 302 | No protein | Processed transcript | - | - | - | TSL:3, |
| ADAR-222 | ENST00000679465.1 | 7272 | No protein | Retained intron | - | - | - | - |
| ADAR-232 | ENST00000681786.1 | 7272 | No protein | Retained intron | - | - | - | - |
| ADAR-223 | ENST00000679805.1 | 7146 | No protein | Retained intron | - | - | - | - |
| ADAR-215 | ENST00000649021.1 | 7112 | No protein | Retained intron | - | - | - | - |
| ADAR-210 | ENST00000647682.2 | 6777 | No protein | Retained intron | - | - | - | - |
| ADAR-230 | ENST00000681429.1 | 6078 | No protein | Retained intron | - | - | - | - |
| ADAR-205 | ENST00000492630.2 | 5203 | No protein | Retained intron | - | - | - | TSL:2, |
| ADAR-227 | ENST00000680472.1 | 2477 | No protein | Retained intron | - | - | - | - |
| ADAR-203 | ENST00000463920.5 | 2347 | No protein | Retained intron | - | - | - | TSL:2, |
| ADAR-209 | ENST00000534279.1 | 849 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ADAR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ADAR had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: