CRISPR-U™ Gene Knockout Cell Line Strategy

CEACAM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CEACAM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CEACAM1
Gene id 634
Organism Homo sapiens
Official full symbol CEA cell adhesion molecule 1
Gene type protein-coding
Also known as BGP, BGP1, BGPI
Summary This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined.
Genomic regions Chromosome 19
Strategy Summary
This gene has 9 protein coding transcripts：
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CEACAM1-201 ENST00000161559.11 3491 526aa Protein coding CCDS12609 P13688-1 NM_001712.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CEACAM1-207 ENST00000403444.7 3427 464aa Protein coding CCDS46089 P13688-8 - TSL:1, GENCODE basic, APPRIS ALT2,
CEACAM1-203 ENST00000352591.9 3170 430aa Protein coding CCDS54273 P13688-6 - TSL:1, GENCODE basic,
CEACAM1-204 ENST00000358394.7 1484 461aa Protein coding CCDS54274 P13688-5 - TSL:1, GENCODE basic,
CEACAM1-208 ENST00000403461.5 1263 368aa Protein coding CCDS54272 P13688-11 - TSL:1, GENCODE basic,
CEACAM1-213 ENST00000599389.1 1342 399aa Protein coding - P13688-9 - TSL:5, GENCODE basic,
CEACAM1-214 ENST00000600172.1 434 99aa Protein coding - M0R109 - CDS 3' incomplete, TSL:5,
CEACAM1-210 ENST00000471298.2 417 94aa Protein coding - M0R2K4 - CDS 3' incomplete, TSL:5,
CEACAM1-215 ENST00000676851.1 161 54aa Protein coding - - - CDS 5' and 3' incomplete,
CEACAM1-212 ENST00000488639.6 429 No protein Processed transcript - - - TSL:3,
CEACAM1-206 ENST00000403136.1 1778 No protein Retained intron - - - TSL:1,
CEACAM1-205 ENST00000377806.7 1773 No protein Retained intron - - - TSL:1,
CEACAM1-202 ENST00000344391.9 1619 No protein Retained intron - - - TSL:1,
CEACAM1-211 ENST00000485605.1 678 No protein Retained intron - - - TSL:5,
CEACAM1-209 ENST00000465051.1 321 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CEACAM1
This KO Strategy loading
Red Cotton™ Notes Gene CEACAM1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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