CRISPR-U™ Gene Knockout Cell Line Strategy

TLE1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TLE1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TLE1
Gene id 7088
Organism Homo sapiens
Official full symbol TLE family member 1, transcriptional corepressor
Gene type protein-coding
Also known as ESG, ESG1, GRG1
Genomic regions Chromosome 9
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TLE1-203 ENST00000376499.8 4135 770aa Protein coding CCDS6661 Q04724 NM_005077.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TLE1-201 ENST00000376463.3 1115 170aa Protein coding - Q5T3G2 - CDS 5' incomplete, TSL:2,
TLE1-204 ENST00000418319.5 936 242aa Protein coding - Q5T3G3 - CDS 3' incomplete, TSL:5,
TLE1-202 ENST00000376484.2 665 146aa Protein coding - F6T2C8 - CDS 5' incomplete, TSL:3,
TLE1-205 ENST00000464999.1 628 209aa Protein coding - A0A669KAE5 - CDS 5' and 3' incomplete, TSL:3,
TLE1-206 ENST00000491534.5 578 192aa Protein coding - A0A669KBK8 - CDS 5' and 3' incomplete, TSL:5,
TLE1-207 ENST00000674113.1 282 41aa Nonsense mediated decay - A0A669KBB1 - CDS 5' incomplete,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TLE1
This KO Strategy loading
Red Cotton™ Notes Gene TLE1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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