CRISPR-U™ Gene Knockout Cell Line
Strategy
TLE1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TLE1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TLE1 |
| Gene id | 7088 |
| Organism | Homo sapiens |
| Official full symbol | TLE family member 1, transcriptional corepressor |
| Gene type | protein-coding |
| Also known as | ESG, ESG1, GRG1 |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TLE1-203 | ENST00000376499.8 | 4135 | 770aa | Protein coding | CCDS6661 | Q04724 | NM_005077.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TLE1-201 | ENST00000376463.3 | 1115 | 170aa | Protein coding | - | Q5T3G2 | - | CDS 5' incomplete, TSL:2, |
| TLE1-204 | ENST00000418319.5 | 936 | 242aa | Protein coding | - | Q5T3G3 | - | CDS 3' incomplete, TSL:5, |
| TLE1-202 | ENST00000376484.2 | 665 | 146aa | Protein coding | - | F6T2C8 | - | CDS 5' incomplete, TSL:3, |
| TLE1-205 | ENST00000464999.1 | 628 | 209aa | Protein coding | - | A0A669KAE5 | - | CDS 5' and 3' incomplete, TSL:3, |
| TLE1-206 | ENST00000491534.5 | 578 | 192aa | Protein coding | - | A0A669KBK8 | - | CDS 5' and 3' incomplete, TSL:5, |
| TLE1-207 | ENST00000674113.1 | 282 | 41aa | Nonsense mediated decay | - | A0A669KBB1 | - | CDS 5' incomplete, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TLE1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TLE1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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