CRISPR-U™ Gene Knockout Cell Line
Strategy
ANXA6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ANXA6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ANXA6 |
| Gene id | 309 |
| Organism | Homo sapiens |
| Official full symbol | annexin A6 |
| Gene type | protein-coding |
| Also known as | ANX6, CBP68, CPB-II, p68, p70 |
| Summary | Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ANXA6-201 | ENST00000354546.10 | 2889 | 673aa | Protein coding | CCDS47315 | P08133-1 | NM_001155.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ANXA6-215 | ENST00000523714.5 | 2451 | 641aa | Protein coding | CCDS54941 | P08133-2 | - | TSL:2, GENCODE basic, |
| ANXA6-211 | ENST00000521512.5 | 1793 | 460aa | Protein coding | - | E5RK69 | - | TSL:5, GENCODE basic, |
| ANXA6-202 | ENST00000377751.9 | 1430 | 330aa | Protein coding | - | E7EMC6 | - | TSL:2, GENCODE basic, |
| ANXA6-214 | ENST00000523164.1 | 923 | 110aa | Protein coding | - | E5RJR0 | - | CDS 3' incomplete, TSL:5, |
| ANXA6-212 | ENST00000521749.5 | 880 | 150aa | Protein coding | - | E5RK63 | - | CDS 3' incomplete, TSL:3, |
| ANXA6-203 | ENST00000517486.5 | 562 | 156aa | Protein coding | - | E5RFF0 | - | CDS 3' incomplete, TSL:5, |
| ANXA6-206 | ENST00000517757.5 | 553 | 129aa | Protein coding | - | E5RJF5 | - | CDS 3' incomplete, TSL:4, |
| ANXA6-213 | ENST00000522664.5 | 451 | 91aa | Protein coding | - | H0YC77 | - | CDS 5' incomplete, TSL:5, |
| ANXA6-204 | ENST00000517677.5 | 795 | 129aa | Nonsense mediated decay | - | E5RI05 | - | TSL:5, |
| ANXA6-208 | ENST00000519644.6 | 728 | 95aa | Nonsense mediated decay | - | E5RIU8 | - | TSL:5, |
| ANXA6-207 | ENST00000519610.1 | 899 | No protein | Processed transcript | - | - | - | TSL:2, |
| ANXA6-210 | ENST00000520378.1 | 1119 | No protein | Retained intron | - | - | - | TSL:5, |
| ANXA6-205 | ENST00000517707.5 | 603 | No protein | Retained intron | - | - | - | TSL:2, |
| ANXA6-209 | ENST00000520054.1 | 487 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ANXA6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ANXA6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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