CRISPR-U™ Gene Knockout Cell Line Strategy

ANXA6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ANXA6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ANXA6
Gene id 309
Organism Homo sapiens
Official full symbol annexin A6
Gene type protein-coding
Also known as ANX6, CBP68, CPB-II, p68, p70
Summary Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described.
Genomic regions Chromosome 5
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ANXA6-201 ENST00000354546.10 2889 673aa Protein coding CCDS47315 P08133-1 NM_001155.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ANXA6-215 ENST00000523714.5 2451 641aa Protein coding CCDS54941 P08133-2 - TSL:2, GENCODE basic,
ANXA6-211 ENST00000521512.5 1793 460aa Protein coding - E5RK69 - TSL:5, GENCODE basic,
ANXA6-202 ENST00000377751.9 1430 330aa Protein coding - E7EMC6 - TSL:2, GENCODE basic,
ANXA6-214 ENST00000523164.1 923 110aa Protein coding - E5RJR0 - CDS 3' incomplete, TSL:5,
ANXA6-212 ENST00000521749.5 880 150aa Protein coding - E5RK63 - CDS 3' incomplete, TSL:3,
ANXA6-203 ENST00000517486.5 562 156aa Protein coding - E5RFF0 - CDS 3' incomplete, TSL:5,
ANXA6-206 ENST00000517757.5 553 129aa Protein coding - E5RJF5 - CDS 3' incomplete, TSL:4,
ANXA6-213 ENST00000522664.5 451 91aa Protein coding - H0YC77 - CDS 5' incomplete, TSL:5,
ANXA6-204 ENST00000517677.5 795 129aa Nonsense mediated decay - E5RI05 - TSL:5,
ANXA6-208 ENST00000519644.6 728 95aa Nonsense mediated decay - E5RIU8 - TSL:5,
ANXA6-207 ENST00000519610.1 899 No protein Processed transcript - - - TSL:2,
ANXA6-210 ENST00000520378.1 1119 No protein Retained intron - - - TSL:5,
ANXA6-205 ENST00000517707.5 603 No protein Retained intron - - - TSL:2,
ANXA6-209 ENST00000520054.1 487 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ANXA6
This KO Strategy loading
Red Cotton™ Notes Gene ANXA6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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