CRISPR-U™ Gene Knockout Cell Line Strategy

CDC42 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDC42 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDC42
Gene id 998
Organism Homo sapiens
Official full symbol cell division cycle 42
Gene type protein-coding
Also known as CDC42Hs, G25K, TKS
Summary The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20.
Genomic regions Chromosome 1
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDC42-206 ENST00000648594.1 10598 191aa Protein coding CCDS221 P60953-2 - GENCODE basic, APPRIS P3,
CDC42-209 ENST00000656825.1 10503 191aa Protein coding CCDS221 P60953-2 NM_001791.4 GENCODE basic, APPRIS P3, MANE Select v0.92,
CDC42-203 ENST00000400259.5 2274 191aa Protein coding CCDS221 P60953-2 - TSL:5, GENCODE basic, APPRIS P3,
CDC42-202 ENST00000344548.7 2256 191aa Protein coding CCDS221 P60953-2 - TSL:1, GENCODE basic, APPRIS P3,
CDC42-210 ENST00000662562.1 1645 191aa Protein coding CCDS221 P60953-2 - GENCODE basic, APPRIS P3,
CDC42-201 ENST00000315554.13 1435 191aa Protein coding CCDS222 P60953-1 - TSL:1, GENCODE basic, APPRIS ALT1,
CDC42-211 ENST00000667384.1 4466 166aa Protein coding - A0A590UJK8 - GENCODE basic,
CDC42-204 ENST00000411827.1 597 136aa Protein coding - Q5JYX0 - CDS 3' incomplete, TSL:3,
CDC42-208 ENST00000652582.1 551 156aa Protein coding - A0A494BZX6 - CDS 3' incomplete,
CDC42-207 ENST00000651171.1 526 115aa Protein coding - A0A494C1M1 - CDS 3' incomplete,
CDC42-205 ENST00000498236.1 816 41aa Nonsense mediated decay - A0A3B3IRV0 - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDC42
This KO Strategy loading
Red Cotton™ Notes Gene CDC42 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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