CRISPR-U™ Gene Knockout Cell Line
Strategy
CDC42 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDC42 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDC42 |
| Gene id | 998 |
| Organism | Homo sapiens |
| Official full symbol | cell division cycle 42 |
| Gene type | protein-coding |
| Also known as | CDC42Hs, G25K, TKS |
| Summary | The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDC42-206 | ENST00000648594.1 | 10598 | 191aa | Protein coding | CCDS221 | P60953-2 | - | GENCODE basic, APPRIS P3, |
| CDC42-209 | ENST00000656825.1 | 10503 | 191aa | Protein coding | CCDS221 | P60953-2 | NM_001791.4 | GENCODE basic, APPRIS P3, MANE Select v0.92, |
| CDC42-203 | ENST00000400259.5 | 2274 | 191aa | Protein coding | CCDS221 | P60953-2 | - | TSL:5, GENCODE basic, APPRIS P3, |
| CDC42-202 | ENST00000344548.7 | 2256 | 191aa | Protein coding | CCDS221 | P60953-2 | - | TSL:1, GENCODE basic, APPRIS P3, |
| CDC42-210 | ENST00000662562.1 | 1645 | 191aa | Protein coding | CCDS221 | P60953-2 | - | GENCODE basic, APPRIS P3, |
| CDC42-201 | ENST00000315554.13 | 1435 | 191aa | Protein coding | CCDS222 | P60953-1 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| CDC42-211 | ENST00000667384.1 | 4466 | 166aa | Protein coding | - | A0A590UJK8 | - | GENCODE basic, |
| CDC42-204 | ENST00000411827.1 | 597 | 136aa | Protein coding | - | Q5JYX0 | - | CDS 3' incomplete, TSL:3, |
| CDC42-208 | ENST00000652582.1 | 551 | 156aa | Protein coding | - | A0A494BZX6 | - | CDS 3' incomplete, |
| CDC42-207 | ENST00000651171.1 | 526 | 115aa | Protein coding | - | A0A494C1M1 | - | CDS 3' incomplete, |
| CDC42-205 | ENST00000498236.1 | 816 | 41aa | Nonsense mediated decay | - | A0A3B3IRV0 | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDC42 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDC42 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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