CRISPR-U™ Gene Knockout Cell Line
Strategy
AK2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human AK2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | AK2 |
| Gene id | 204 |
| Organism | Homo sapiens |
| Official full symbol | adenylate kinase 2 |
| Gene type | protein-coding |
| Also known as | ADK2 |
| Summary | Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| AK2-217 | ENST00000672715.1 | 5970 | 239aa | Protein coding | CCDS374 | P54819-1 | NM_001625.4 | GENCODE basic, APPRIS P4, MANE Select v0.92, |
| AK2-202 | ENST00000373449.7 | 3597 | 232aa | Protein coding | CCDS373 | P54819-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| AK2-215 | ENST00000629371.2 | 3576 | 133aa | Protein coding | CCDS81295 | G3V213 | - | TSL:3, GENCODE basic, |
| AK2-210 | ENST00000548033.5 | 936 | 190aa | Protein coding | CCDS81294 | F8VY04 | - | TSL:1, GENCODE basic, |
| AK2-204 | ENST00000467905.5 | 880 | 232aa | Protein coding | CCDS81296 | F8W1A4 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| AK2-206 | ENST00000480134.5 | 811 | 133aa | Protein coding | CCDS81295 | G3V213 | - | TSL:2, GENCODE basic, |
| AK2-201 | ENST00000354858.11 | 5059 | 197aa | Protein coding | - | A0A5K1VW67 | - | TSL:1, GENCODE basic, |
| AK2-218 | ENST00000673291.1 | 3040 | 162aa | Protein coding | - | A0A5F9ZHP2 | - | GENCODE basic, |
| AK2-208 | ENST00000487289.1 | 920 | 162aa | Protein coding | - | F8VZG5 | - | TSL:2, GENCODE basic, |
| AK2-214 | ENST00000626911.1 | 160 | 42aa | Protein coding | - | F8VPP1 | - | TSL:3, GENCODE basic, |
| AK2-212 | ENST00000550338.5 | 886 | 42aa | Nonsense mediated decay | - | F8VPP1 | - | TSL:2, |
| AK2-209 | ENST00000491241.1 | 596 | No protein | Processed transcript | - | - | - | TSL:3, |
| AK2-211 | ENST00000548559.1 | 530 | No protein | Processed transcript | - | - | - | TSL:4, |
| AK2-216 | ENST00000672308.1 | 2840 | No protein | Retained intron | - | - | - | - |
| AK2-207 | ENST00000482191.1 | 599 | No protein | Retained intron | - | - | - | TSL:2, |
| AK2-213 | ENST00000551979.1 | 581 | No protein | Retained intron | - | - | - | TSL:2, |
| AK2-205 | ENST00000469238.1 | 423 | No protein | Retained intron | - | - | - | TSL:2, |
| AK2-203 | ENST00000466029.1 | 223 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | AK2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene AK2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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