CRISPR-U™ Gene Knockout Cell Line Strategy

AK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human AK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol AK2
Gene id 204
Organism Homo sapiens
Official full symbol adenylate kinase 2
Gene type protein-coding
Also known as ADK2
Summary Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.
Genomic regions Chromosome 1
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
AK2-217 ENST00000672715.1 5970 239aa Protein coding CCDS374 P54819-1 NM_001625.4 GENCODE basic, APPRIS P4, MANE Select v0.92,
AK2-202 ENST00000373449.7 3597 232aa Protein coding CCDS373 P54819-2 - TSL:1, GENCODE basic, APPRIS ALT1,
AK2-215 ENST00000629371.2 3576 133aa Protein coding CCDS81295 G3V213 - TSL:3, GENCODE basic,
AK2-210 ENST00000548033.5 936 190aa Protein coding CCDS81294 F8VY04 - TSL:1, GENCODE basic,
AK2-204 ENST00000467905.5 880 232aa Protein coding CCDS81296 F8W1A4 - TSL:2, GENCODE basic, APPRIS ALT1,
AK2-206 ENST00000480134.5 811 133aa Protein coding CCDS81295 G3V213 - TSL:2, GENCODE basic,
AK2-201 ENST00000354858.11 5059 197aa Protein coding - A0A5K1VW67 - TSL:1, GENCODE basic,
AK2-218 ENST00000673291.1 3040 162aa Protein coding - A0A5F9ZHP2 - GENCODE basic,
AK2-208 ENST00000487289.1 920 162aa Protein coding - F8VZG5 - TSL:2, GENCODE basic,
AK2-214 ENST00000626911.1 160 42aa Protein coding - F8VPP1 - TSL:3, GENCODE basic,
AK2-212 ENST00000550338.5 886 42aa Nonsense mediated decay - F8VPP1 - TSL:2,
AK2-209 ENST00000491241.1 596 No protein Processed transcript - - - TSL:3,
AK2-211 ENST00000548559.1 530 No protein Processed transcript - - - TSL:4,
AK2-216 ENST00000672308.1 2840 No protein Retained intron - - - -
AK2-207 ENST00000482191.1 599 No protein Retained intron - - - TSL:2,
AK2-213 ENST00000551979.1 581 No protein Retained intron - - - TSL:2,
AK2-205 ENST00000469238.1 423 No protein Retained intron - - - TSL:2,
AK2-203 ENST00000466029.1 223 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene AK2
This KO Strategy loading
Red Cotton™ Notes Gene AK2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×