CRISPR-U™ Gene Knockout Cell Line
Strategy
DEGS1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DEGS1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DEGS1 |
| Gene id | 8560 |
| Organism | Homo sapiens |
| Official full symbol | delta 4-desaturase, sphingolipid 1 |
| Gene type | protein-coding |
| Also known as | DEGS, DEGS-1, DES1, Des-1, FADS7, HLD18, MIG15, MLD |
| Summary | This gene encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DEGS1-201 | ENST00000323699.9 | 2032 | 323aa | Protein coding | CCDS1540 | A0A024R3P1 O15121 | NM_003676.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DEGS1-202 | ENST00000391877.3 | 1345 | 323aa | Protein coding | CCDS1540 | A0A024R3P1 O15121 | - | TSL:5, GENCODE basic, APPRIS P1, |
| DEGS1-203 | ENST00000415210.5 | 957 | 261aa | Protein coding | - | E7EMA0 | - | CDS 3' incomplete, TSL:5, |
| DEGS1-204 | ENST00000465848.1 | 633 | No protein | Processed transcript | - | - | - | TSL:2, |
| DEGS1-205 | ENST00000498813.1 | 358 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DEGS1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DEGS1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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