CRISPR-U™ Gene Knockout Cell Line Strategy

DEGS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DEGS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DEGS1
Gene id 8560
Organism Homo sapiens
Official full symbol delta 4-desaturase, sphingolipid 1
Gene type protein-coding
Also known as DEGS, DEGS-1, DES1, Des-1, FADS7, HLD18, MIG15, MLD
Summary This gene encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DEGS1-201 ENST00000323699.9 2032 323aa Protein coding CCDS1540 A0A024R3P1 O15121 NM_003676.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DEGS1-202 ENST00000391877.3 1345 323aa Protein coding CCDS1540 A0A024R3P1 O15121 - TSL:5, GENCODE basic, APPRIS P1,
DEGS1-203 ENST00000415210.5 957 261aa Protein coding - E7EMA0 - CDS 3' incomplete, TSL:5,
DEGS1-204 ENST00000465848.1 633 No protein Processed transcript - - - TSL:2,
DEGS1-205 ENST00000498813.1 358 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DEGS1
This KO Strategy loading
Red Cotton™ Notes Gene DEGS1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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