CRISPR-U™ Gene Knockout Cell Line
Strategy
ABCC1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ABCC1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ABCC1 |
| Gene id | 4363 |
| Organism | Homo sapiens |
| Official full symbol | ATP binding cassette subfamily C member 1 |
| Gene type | protein-coding |
| Also known as | ABC29, ABCC, DFNA77, GS-X, MRP, MRP1 |
| Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ABCC1-202 | ENST00000399410.8 | 6504 | 1531aa | Protein coding | CCDS42122 | P33527-1 | NM_004996.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ABCC1-201 | ENST00000399408.7 | 6594 | 1541aa | Protein coding | - | A0A0A0MS99 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| ABCC1-203 | ENST00000572882.3 | 6387 | 1472aa | Protein coding | - | I3L4X2 | - | TSL:1, |
| ABCC1-209 | ENST00000677164.1 | 6276 | 1374aa | Protein coding | - | - | - | GENCODE basic, |
| ABCC1-210 | ENST00000678422.1 | 6445 | 866aa | Nonsense mediated decay | - | - | - | - |
| ABCC1-208 | ENST00000676806.1 | 3077 | No protein | Processed transcript | - | - | - | - |
| ABCC1-211 | ENST00000679043.1 | 1770 | No protein | Processed transcript | - | - | - | - |
| ABCC1-207 | ENST00000576557.1 | 542 | No protein | Processed transcript | - | - | - | TSL:4, |
| ABCC1-204 | ENST00000574224.2 | 2616 | No protein | Retained intron | - | - | - | TSL:1, |
| ABCC1-206 | ENST00000575422.5 | 1198 | No protein | Retained intron | - | - | - | TSL:5, |
| ABCC1-205 | ENST00000574761.1 | 996 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ABCC1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ABCC1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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