CRISPR-U™ Gene Knockout Cell Line Strategy

ABCC1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ABCC1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ABCC1
Gene id 4363
Organism Homo sapiens
Official full symbol ATP binding cassette subfamily C member 1
Gene type protein-coding
Also known as ABC29, ABCC, DFNA77, GS-X, MRP, MRP1
Summary The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.
Genomic regions Chromosome 16
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ABCC1-202 ENST00000399410.8 6504 1531aa Protein coding CCDS42122 P33527-1 NM_004996.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
ABCC1-201 ENST00000399408.7 6594 1541aa Protein coding - A0A0A0MS99 - TSL:5, GENCODE basic, APPRIS ALT2,
ABCC1-203 ENST00000572882.3 6387 1472aa Protein coding - I3L4X2 - TSL:1,
ABCC1-209 ENST00000677164.1 6276 1374aa Protein coding - - - GENCODE basic,
ABCC1-210 ENST00000678422.1 6445 866aa Nonsense mediated decay - - - -
ABCC1-208 ENST00000676806.1 3077 No protein Processed transcript - - - -
ABCC1-211 ENST00000679043.1 1770 No protein Processed transcript - - - -
ABCC1-207 ENST00000576557.1 542 No protein Processed transcript - - - TSL:4,
ABCC1-204 ENST00000574224.2 2616 No protein Retained intron - - - TSL:1,
ABCC1-206 ENST00000575422.5 1198 No protein Retained intron - - - TSL:5,
ABCC1-205 ENST00000574761.1 996 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ABCC1
This KO Strategy loading
Red Cotton™ Notes Gene ABCC1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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