CRISPR-U™ Gene Knockout Cell Line
Strategy
HIBADH Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HIBADH Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HIBADH |
| Gene id | 11112 |
| Organism | Homo sapiens |
| Official full symbol | 3-hydroxyisobutyrate dehydrogenase |
| Gene type | protein-coding |
| Also known as | NS5ATP1 |
| Summary | This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HIBADH-201 | ENST00000265395.7 | 1878 | 336aa | Protein coding | CCDS5414 | A0A024RA75 P31937 | NM_152740.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HIBADH-202 | ENST00000425715.1 | 618 | 206aa | Protein coding | - | H7BZL2 | - | CDS 5' and 3' incomplete, TSL:2, |
| HIBADH-203 | ENST00000428288.2 | 1669 | 39aa | Nonsense mediated decay | - | F8WET2 | - | TSL:3, |
| HIBADH-204 | ENST00000496814.1 | 570 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HIBADH |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HIBADH had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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