CRISPR-U™ Gene Knockout Cell Line Strategy

HIBADH Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HIBADH Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HIBADH
Gene id 11112
Organism Homo sapiens
Official full symbol 3-hydroxyisobutyrate dehydrogenase
Gene type protein-coding
Also known as NS5ATP1
Summary This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.
Genomic regions Chromosome 7
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HIBADH-201 ENST00000265395.7 1878 336aa Protein coding CCDS5414 A0A024RA75 P31937 NM_152740.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HIBADH-202 ENST00000425715.1 618 206aa Protein coding - H7BZL2 - CDS 5' and 3' incomplete, TSL:2,
HIBADH-203 ENST00000428288.2 1669 39aa Nonsense mediated decay - F8WET2 - TSL:3,
HIBADH-204 ENST00000496814.1 570 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HIBADH
This KO Strategy loading
Red Cotton™ Notes Gene HIBADH had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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