CRISPR-U™ Gene Knockout Cell Line
Strategy
CPS1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CPS1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CPS1 |
| Gene id | 1373 |
| Organism | Homo sapiens |
| Official full symbol | carbamoyl-phosphate synthase 1 |
| Gene type | protein-coding |
| Also known as | CPSASE1, PHN |
| Summary | The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CPS1-213 | ENST00000673630.1 | 5775 | 1500aa | Protein coding | CCDS2393 | P31327-1 | - | GENCODE basic, APPRIS P1, |
| CPS1-201 | ENST00000233072.10 | 5760 | 1500aa | Protein coding | CCDS2393 | P31327-1 | NM_001875.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CPS1-202 | ENST00000430249.7 | 5723 | 1506aa | Protein coding | CCDS46505 | P31327-3 | - | TSL:1, GENCODE basic, |
| CPS1-215 | ENST00000673711.1 | 5698 | 1500aa | Protein coding | CCDS2393 | P31327-1 | - | GENCODE basic, APPRIS P1, |
| CPS1-212 | ENST00000673510.1 | 4880 | 1500aa | Protein coding | CCDS2393 | P31327-1 | - | GENCODE basic, APPRIS P1, |
| CPS1-203 | ENST00000451903.3 | 4779 | 1049aa | Protein coding | CCDS46506 | P31327-2 | - | TSL:1, GENCODE basic, |
| CPS1-209 | ENST00000619804.1 | 1542 | 513aa | Protein coding | - | Q5R211 | - | TSL:5, GENCODE basic, |
| CPS1-214 | ENST00000673698.1 | 4138 | 660aa | Nonsense mediated decay | - | A0A669KBF2 | - | CDS 5' incomplete, |
| CPS1-216 | ENST00000674074.1 | 4770 | No protein | Processed transcript | - | - | - | - |
| CPS1-210 | ENST00000645825.1 | 2180 | No protein | Processed transcript | - | - | - | - |
| CPS1-207 | ENST00000497121.1 | 585 | No protein | Processed transcript | - | - | - | TSL:4, |
| CPS1-211 | ENST00000671984.1 | 501 | No protein | Processed transcript | - | - | - | - |
| CPS1-206 | ENST00000479988.1 | 4811 | No protein | Retained intron | - | - | - | TSL:1, |
| CPS1-205 | ENST00000470791.1 | 501 | No protein | Retained intron | - | - | - | TSL:2, |
| CPS1-208 | ENST00000497163.1 | 443 | No protein | Retained intron | - | - | - | TSL:3, |
| CPS1-204 | ENST00000467353.1 | 323 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CPS1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CPS1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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