CRISPR-U™ Gene Knockout Cell Line Strategy

CPS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CPS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CPS1
Gene id 1373
Organism Homo sapiens
Official full symbol carbamoyl-phosphate synthase 1
Gene type protein-coding
Also known as CPSASE1, PHN
Summary The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.
Genomic regions Chromosome 2
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CPS1-213 ENST00000673630.1 5775 1500aa Protein coding CCDS2393 P31327-1 - GENCODE basic, APPRIS P1,
CPS1-201 ENST00000233072.10 5760 1500aa Protein coding CCDS2393 P31327-1 NM_001875.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CPS1-202 ENST00000430249.7 5723 1506aa Protein coding CCDS46505 P31327-3 - TSL:1, GENCODE basic,
CPS1-215 ENST00000673711.1 5698 1500aa Protein coding CCDS2393 P31327-1 - GENCODE basic, APPRIS P1,
CPS1-212 ENST00000673510.1 4880 1500aa Protein coding CCDS2393 P31327-1 - GENCODE basic, APPRIS P1,
CPS1-203 ENST00000451903.3 4779 1049aa Protein coding CCDS46506 P31327-2 - TSL:1, GENCODE basic,
CPS1-209 ENST00000619804.1 1542 513aa Protein coding - Q5R211 - TSL:5, GENCODE basic,
CPS1-214 ENST00000673698.1 4138 660aa Nonsense mediated decay - A0A669KBF2 - CDS 5' incomplete,
CPS1-216 ENST00000674074.1 4770 No protein Processed transcript - - - -
CPS1-210 ENST00000645825.1 2180 No protein Processed transcript - - - -
CPS1-207 ENST00000497121.1 585 No protein Processed transcript - - - TSL:4,
CPS1-211 ENST00000671984.1 501 No protein Processed transcript - - - -
CPS1-206 ENST00000479988.1 4811 No protein Retained intron - - - TSL:1,
CPS1-205 ENST00000470791.1 501 No protein Retained intron - - - TSL:2,
CPS1-208 ENST00000497163.1 443 No protein Retained intron - - - TSL:3,
CPS1-204 ENST00000467353.1 323 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CPS1
This KO Strategy loading
Red Cotton™ Notes Gene CPS1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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