CRISPR-U™ Gene Knockout Cell Line
Strategy
BCL6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BCL6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BCL6 |
| Gene id | 604 |
| Organism | Homo sapiens |
| Official full symbol | BCL6 transcription repressor |
| Gene type | protein-coding |
| Also known as | BCL5, BCL6A, LAZ3, ZBTB27, ZNF51 |
| Summary | The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BCL6-211 | ENST00000621333.4 | 3399 | 650aa | Protein coding | CCDS46975 | P41182-2 | - | TSL:5, GENCODE basic, |
| BCL6-202 | ENST00000406870.7 | 3306 | 706aa | Protein coding | CCDS3289 | P41182-1 | NM_001706.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BCL6-201 | ENST00000232014.8 | 2710 | 706aa | Protein coding | CCDS3289 | P41182-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| BCL6-206 | ENST00000450123.6 | 2003 | 650aa | Protein coding | CCDS46975 | P41182-2 | - | TSL:1, GENCODE basic, |
| BCL6-205 | ENST00000438077.1 | 709 | 104aa | Protein coding | - | C9JL16 | - | CDS 3' incomplete, TSL:3, |
| BCL6-204 | ENST00000430339.5 | 454 | 121aa | Protein coding | - | C9JCS5 | - | CDS 3' incomplete, TSL:2, |
| BCL6-203 | ENST00000419510.6 | 4178 | 55aa | Nonsense mediated decay | - | A0A0C4DH53 | - | TSL:2, |
| BCL6-210 | ENST00000496823.1 | 1138 | No protein | Processed transcript | - | - | - | TSL:3, |
| BCL6-209 | ENST00000480458.5 | 342 | No protein | Processed transcript | - | - | - | TSL:5, |
| BCL6-208 | ENST00000479110.1 | 635 | No protein | Retained intron | - | - | - | TSL:2, |
| BCL6-207 | ENST00000470319.1 | 526 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BCL6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BCL6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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