CRISPR-U™ Gene Knockout Cell Line Strategy

BCL6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BCL6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BCL6
Gene id 604
Organism Homo sapiens
Official full symbol BCL6 transcription repressor
Gene type protein-coding
Also known as BCL5, BCL6A, LAZ3, ZBTB27, ZNF51
Summary The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.
Genomic regions Chromosome 3
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BCL6-211 ENST00000621333.4 3399 650aa Protein coding CCDS46975 P41182-2 - TSL:5, GENCODE basic,
BCL6-202 ENST00000406870.7 3306 706aa Protein coding CCDS3289 P41182-1 NM_001706.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BCL6-201 ENST00000232014.8 2710 706aa Protein coding CCDS3289 P41182-1 - TSL:1, GENCODE basic, APPRIS P1,
BCL6-206 ENST00000450123.6 2003 650aa Protein coding CCDS46975 P41182-2 - TSL:1, GENCODE basic,
BCL6-205 ENST00000438077.1 709 104aa Protein coding - C9JL16 - CDS 3' incomplete, TSL:3,
BCL6-204 ENST00000430339.5 454 121aa Protein coding - C9JCS5 - CDS 3' incomplete, TSL:2,
BCL6-203 ENST00000419510.6 4178 55aa Nonsense mediated decay - A0A0C4DH53 - TSL:2,
BCL6-210 ENST00000496823.1 1138 No protein Processed transcript - - - TSL:3,
BCL6-209 ENST00000480458.5 342 No protein Processed transcript - - - TSL:5,
BCL6-208 ENST00000479110.1 635 No protein Retained intron - - - TSL:2,
BCL6-207 ENST00000470319.1 526 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BCL6
This KO Strategy loading
Red Cotton™ Notes Gene BCL6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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