CRISPR-U™ Gene Knockout Cell Line
Strategy
CAMK2D Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CAMK2D Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CAMK2D |
| Gene id | 817 |
| Organism | Homo sapiens |
| Official full symbol | calcium/calmodulin dependent protein kinase II delta |
| Gene type | protein-coding |
| Also known as | CAMKD |
| Summary | The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CAMK2D-201 | ENST00000296402.9 | 5820 | 478aa | Protein coding | CCDS3704 | Q13557-8 | - | TSL:5, GENCODE basic, |
| CAMK2D-211 | ENST00000511664.6 | 5785 | 533aa | Protein coding | CCDS82949 | E9PF82 | NM_001321571.2 | TSL:2, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| CAMK2D-205 | ENST00000394524.7 | 5294 | 478aa | Protein coding | CCDS43263 | Q13557-12 | - | TSL:2, GENCODE basic, |
| CAMK2D-204 | ENST00000394522.7 | 2696 | 492aa | Protein coding | CCDS47127 | Q13557-10 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| CAMK2D-202 | ENST00000342666.9 | 1940 | 499aa | Protein coding | CCDS3703 | Q13557-1 | - | TSL:5, GENCODE basic, |
| CAMK2D-207 | ENST00000505990.5 | 1802 | 512aa | Protein coding | CCDS82948 | E9PBG7 | - | TSL:5, GENCODE basic, APPRIS P4, |
| CAMK2D-203 | ENST00000379773.6 | 1642 | 478aa | Protein coding | CCDS3704 | Q13557-8 | - | TSL:1, GENCODE basic, |
| CAMK2D-213 | ENST00000514328.5 | 1497 | 498aa | Protein coding | CCDS82950 | D6R938 | - | TSL:5, GENCODE basic, |
| CAMK2D-208 | ENST00000508738.5 | 1470 | 489aa | Protein coding | CCDS54797 | Q13557-9 | - | TSL:1, GENCODE basic, |
| CAMK2D-212 | ENST00000513132.5 | 2142 | 182aa | Protein coding | - | H0Y9J2 | - | CDS 5' incomplete, TSL:2, |
| CAMK2D-214 | ENST00000515496.5 | 1533 | 510aa | Protein coding | - | Q13557-3 | - | TSL:5, GENCODE basic, |
| CAMK2D-209 | ENST00000509594.1 | 583 | 194aa | Protein coding | - | H0Y9C2 | - | CDS 5' and 3' incomplete, TSL:5, |
| CAMK2D-210 | ENST00000509907.1 | 860 | No protein | Processed transcript | - | - | - | TSL:2, |
| CAMK2D-206 | ENST00000505132.1 | 748 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CAMK2D |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CAMK2D had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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