CRISPR-U™ Gene Knockout Cell Line Strategy

CAMK2D Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CAMK2D Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CAMK2D
Gene id 817
Organism Homo sapiens
Official full symbol calcium/calmodulin dependent protein kinase II delta
Gene type protein-coding
Also known as CAMKD
Summary The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.
Genomic regions Chromosome 4
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CAMK2D-201 ENST00000296402.9 5820 478aa Protein coding CCDS3704 Q13557-8 - TSL:5, GENCODE basic,
CAMK2D-211 ENST00000511664.6 5785 533aa Protein coding CCDS82949 E9PF82 NM_001321571.2 TSL:2, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
CAMK2D-205 ENST00000394524.7 5294 478aa Protein coding CCDS43263 Q13557-12 - TSL:2, GENCODE basic,
CAMK2D-204 ENST00000394522.7 2696 492aa Protein coding CCDS47127 Q13557-10 - TSL:1, GENCODE basic, APPRIS ALT1,
CAMK2D-202 ENST00000342666.9 1940 499aa Protein coding CCDS3703 Q13557-1 - TSL:5, GENCODE basic,
CAMK2D-207 ENST00000505990.5 1802 512aa Protein coding CCDS82948 E9PBG7 - TSL:5, GENCODE basic, APPRIS P4,
CAMK2D-203 ENST00000379773.6 1642 478aa Protein coding CCDS3704 Q13557-8 - TSL:1, GENCODE basic,
CAMK2D-213 ENST00000514328.5 1497 498aa Protein coding CCDS82950 D6R938 - TSL:5, GENCODE basic,
CAMK2D-208 ENST00000508738.5 1470 489aa Protein coding CCDS54797 Q13557-9 - TSL:1, GENCODE basic,
CAMK2D-212 ENST00000513132.5 2142 182aa Protein coding - H0Y9J2 - CDS 5' incomplete, TSL:2,
CAMK2D-214 ENST00000515496.5 1533 510aa Protein coding - Q13557-3 - TSL:5, GENCODE basic,
CAMK2D-209 ENST00000509594.1 583 194aa Protein coding - H0Y9C2 - CDS 5' and 3' incomplete, TSL:5,
CAMK2D-210 ENST00000509907.1 860 No protein Processed transcript - - - TSL:2,
CAMK2D-206 ENST00000505132.1 748 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CAMK2D
This KO Strategy loading
Red Cotton™ Notes Gene CAMK2D had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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