CRISPR-U™ Gene Knockout Cell Line
Strategy
MAPK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAPK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAPK1 |
| Gene id | 5594 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase 1 |
| Gene type | protein-coding |
| Also known as | ERK, ERK-2, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK |
| Summary | This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAPK1-201 | ENST00000215832.11 | 5881 | 360aa | Protein coding | CCDS13795 | P28482-1 | NM_002745.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MAPK1-202 | ENST00000398822.7 | 1487 | 360aa | Protein coding | CCDS13795 | P28482-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MAPK1-204 | ENST00000544786.1 | 951 | 316aa | Protein coding | - | P28482-2 | - | TSL:1, GENCODE basic, |
| MAPK1-203 | ENST00000491588.1 | 491 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAPK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAPK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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