CRISPR-U™ Gene Knockout Cell Line
Strategy
GBA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GBA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GBA |
| Gene id | 2629 |
| Organism | Homo sapiens |
| Official full symbol | glucosylceramidase beta |
| Gene type | protein-coding |
| Also known as | GBA1, GCB, GLUC |
| Summary | This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GBA-201 | ENST00000327247.9 | 2387 | 536aa | Protein coding | CCDS1102 | P04062-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| GBA-202 | ENST00000368373.8 | 2291 | 536aa | Protein coding | CCDS1102 | P04062-1 | NM_000157.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GBA-203 | ENST00000427500.7 | 2063 | 487aa | Protein coding | CCDS53374 | P04062-5 | - | TSL:2, GENCODE basic, |
| GBA-204 | ENST00000428024.3 | 1817 | 449aa | Protein coding | CCDS53373 | P04062-4 | - | TSL:2, GENCODE basic, |
| GBA-213 | ENST00000493842.5 | 1020 | No protein | Processed transcript | - | - | - | TSL:5, |
| GBA-210 | ENST00000478472.1 | 1005 | No protein | Processed transcript | - | - | - | TSL:3, |
| GBA-212 | ENST00000491081.5 | 892 | No protein | Processed transcript | - | - | - | TSL:2, |
| GBA-209 | ENST00000473570.5 | 862 | No protein | Processed transcript | - | - | - | TSL:5, |
| GBA-205 | ENST00000460156.1 | 750 | No protein | Processed transcript | - | - | - | TSL:2, |
| GBA-211 | ENST00000484489.5 | 749 | No protein | Processed transcript | - | - | - | TSL:5, |
| GBA-214 | ENST00000497670.5 | 696 | No protein | Processed transcript | - | - | - | TSL:5, |
| GBA-207 | ENST00000467918.5 | 625 | No protein | Processed transcript | - | - | - | TSL:5, |
| GBA-206 | ENST00000464536.1 | 559 | No protein | Processed transcript | - | - | - | TSL:2, |
| GBA-208 | ENST00000470104.1 | 487 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GBA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GBA had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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