CRISPR-U™ Gene Knockout Cell Line
Strategy
DNMT3A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DNMT3A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DNMT3A |
| Gene id | 1788 |
| Organism | Homo sapiens |
| Official full symbol | DNA methyltransferase 3 alpha |
| Gene type | protein-coding |
| Also known as | DNMT3A2, HESJAS, M.HsaIIIA, TBRS |
| Summary | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DNMT3A-201 | ENST00000264709.7 | 9501 | 912aa | Protein coding | CCDS33157 | Q9Y6K1-1 | - | TSL:1, GENCODE basic, APPRIS P3, |
| DNMT3A-202 | ENST00000321117.10 | 9421 | 912aa | Protein coding | CCDS33157 | Q9Y6K1-1 | NM_022552.5 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| DNMT3A-203 | ENST00000380746.8 | 3589 | 723aa | Protein coding | CCDS1718 | Q9Y6K1-2 | - | TSL:1, GENCODE basic, |
| DNMT3A-206 | ENST00000406659.3 | 1775 | 166aa | Protein coding | CCDS46232 | Q9Y6K1-3 | - | TSL:1, GENCODE basic, |
| DNMT3A-205 | ENST00000402667.1 | 2300 | 689aa | Protein coding | - | A0A0C4DG02 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| DNMT3A-204 | ENST00000380756.7 | 4477 | 781aa | Nonsense mediated decay | - | F8WE91 | - | TSL:1, |
| DNMT3A-211 | ENST00000474887.5 | 847 | No protein | Processed transcript | - | - | - | TSL:3, |
| DNMT3A-215 | ENST00000496570.1 | 656 | No protein | Processed transcript | - | - | - | TSL:3, |
| DNMT3A-209 | ENST00000470983.5 | 589 | No protein | Processed transcript | - | - | - | TSL:3, |
| DNMT3A-207 | ENST00000461228.1 | 581 | No protein | Processed transcript | - | - | - | TSL:5, |
| DNMT3A-212 | ENST00000482935.5 | 519 | No protein | Processed transcript | - | - | - | TSL:5, |
| DNMT3A-214 | ENST00000491288.5 | 472 | No protein | Processed transcript | - | - | - | TSL:3, |
| DNMT3A-210 | ENST00000474807.5 | 840 | No protein | Retained intron | - | - | - | TSL:5, |
| DNMT3A-208 | ENST00000466601.5 | 812 | No protein | Retained intron | - | - | - | TSL:2, |
| DNMT3A-213 | ENST00000484184.1 | 619 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DNMT3A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DNMT3A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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