CRISPR-U™ Gene Knockout Cell Line Strategy

DNMT3A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DNMT3A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DNMT3A
Gene id 1788
Organism Homo sapiens
Official full symbol DNA methyltransferase 3 alpha
Gene type protein-coding
Also known as DNMT3A2, HESJAS, M.HsaIIIA, TBRS
Summary CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Genomic regions Chromosome 2
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DNMT3A-201 ENST00000264709.7 9501 912aa Protein coding CCDS33157 Q9Y6K1-1 - TSL:1, GENCODE basic, APPRIS P3,
DNMT3A-202 ENST00000321117.10 9421 912aa Protein coding CCDS33157 Q9Y6K1-1 NM_022552.5 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
DNMT3A-203 ENST00000380746.8 3589 723aa Protein coding CCDS1718 Q9Y6K1-2 - TSL:1, GENCODE basic,
DNMT3A-206 ENST00000406659.3 1775 166aa Protein coding CCDS46232 Q9Y6K1-3 - TSL:1, GENCODE basic,
DNMT3A-205 ENST00000402667.1 2300 689aa Protein coding - A0A0C4DG02 - TSL:5, GENCODE basic, APPRIS ALT1,
DNMT3A-204 ENST00000380756.7 4477 781aa Nonsense mediated decay - F8WE91 - TSL:1,
DNMT3A-211 ENST00000474887.5 847 No protein Processed transcript - - - TSL:3,
DNMT3A-215 ENST00000496570.1 656 No protein Processed transcript - - - TSL:3,
DNMT3A-209 ENST00000470983.5 589 No protein Processed transcript - - - TSL:3,
DNMT3A-207 ENST00000461228.1 581 No protein Processed transcript - - - TSL:5,
DNMT3A-212 ENST00000482935.5 519 No protein Processed transcript - - - TSL:5,
DNMT3A-214 ENST00000491288.5 472 No protein Processed transcript - - - TSL:3,
DNMT3A-210 ENST00000474807.5 840 No protein Retained intron - - - TSL:5,
DNMT3A-208 ENST00000466601.5 812 No protein Retained intron - - - TSL:2,
DNMT3A-213 ENST00000484184.1 619 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DNMT3A
This KO Strategy loading
Red Cotton™ Notes Gene DNMT3A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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