CRISPR-U™ Gene Knockout Cell Line
Strategy
CFH Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CFH Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CFH |
| Gene id | 3075 |
| Organism | Homo sapiens |
| Official full symbol | complement factor H |
| Gene type | protein-coding |
| Also known as | AHUS1, AMBP1, ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS |
| Summary | This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CFH-202 | ENST00000367429.9 | 3962 | 1231aa | Protein coding | CCDS1385 | A0A024R962 P08603 | NM_000186.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CFH-206 | ENST00000630130.2 | 1658 | 449aa | Protein coding | CCDS53452 | A0A0D9SG88 | - | TSL:1, GENCODE basic, |
| CFH-201 | ENST00000359637.2 | 1454 | 385aa | Protein coding | - | Q5TFM2 | - | TSL:1, GENCODE basic, |
| CFH-205 | ENST00000496761.1 | 550 | No protein | Processed transcript | - | - | - | TSL:2, |
| CFH-203 | ENST00000466229.5 | 6985 | No protein | Retained intron | - | - | - | TSL:1, |
| CFH-204 | ENST00000470918.1 | 753 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CFH |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CFH had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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