CRISPR-U™ Gene Knockout Cell Line Strategy

CFH Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CFH Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CFH
Gene id 3075
Organism Homo sapiens
Official full symbol complement factor H
Gene type protein-coding
Also known as AHUS1, AMBP1, ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS
Summary This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CFH-202 ENST00000367429.9 3962 1231aa Protein coding CCDS1385 A0A024R962 P08603 NM_000186.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CFH-206 ENST00000630130.2 1658 449aa Protein coding CCDS53452 A0A0D9SG88 - TSL:1, GENCODE basic,
CFH-201 ENST00000359637.2 1454 385aa Protein coding - Q5TFM2 - TSL:1, GENCODE basic,
CFH-205 ENST00000496761.1 550 No protein Processed transcript - - - TSL:2,
CFH-203 ENST00000466229.5 6985 No protein Retained intron - - - TSL:1,
CFH-204 ENST00000470918.1 753 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CFH
This KO Strategy loading
Red Cotton™ Notes Gene CFH had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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