CRISPR-U™ Gene Knockout Cell Line
Strategy
FLOT1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FLOT1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FLOT1 |
| Gene id | 10211 |
| Organism | Homo sapiens |
| Official full symbol | flotillin 1 |
| Gene type | protein-coding |
| Summary | This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FLOT1-201 | ENST00000376389.8 | 1866 | 427aa | Protein coding | CCDS4688 | O75955-1 | NM_005803.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FLOT1-205 | ENST00000438162.5 | 888 | 252aa | Protein coding | - | A2AB10 | - | CDS 3' incomplete, TSL:5, |
| FLOT1-202 | ENST00000413165.5 | 856 | 238aa | Protein coding | - | A2AB09 | - | CDS 3' incomplete, TSL:5, |
| FLOT1-206 | ENST00000445853.5 | 792 | 235aa | Protein coding | - | A2AB12 | - | CDS 3' incomplete, TSL:1, |
| FLOT1-207 | ENST00000454845.1 | 629 | 185aa | Protein coding | - | A2AB13 | - | CDS 3' incomplete, TSL:3, |
| FLOT1-203 | ENST00000416018.5 | 592 | 192aa | Protein coding | - | A2AB11 | - | CDS 3' incomplete, TSL:3, |
| FLOT1-204 | ENST00000418160.5 | 457 | 145aa | Protein coding | - | A2ABJ5 | - | CDS 3' incomplete, TSL:3, |
| FLOT1-208 | ENST00000470643.5 | 852 | No protein | Processed transcript | - | - | - | TSL:3, |
| FLOT1-212 | ENST00000487376.5 | 1583 | No protein | Retained intron | - | - | - | TSL:5, |
| FLOT1-210 | ENST00000484168.1 | 1101 | No protein | Retained intron | - | - | - | TSL:5, |
| FLOT1-211 | ENST00000484693.1 | 1001 | No protein | Retained intron | - | - | - | TSL:2, |
| FLOT1-209 | ENST00000476729.5 | 562 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FLOT1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FLOT1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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