CRISPR-U™ Gene Knockout Cell Line Strategy

FLOT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FLOT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FLOT1
Gene id 10211
Organism Homo sapiens
Official full symbol flotillin 1
Gene type protein-coding
Summary This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 6
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FLOT1-201 ENST00000376389.8 1866 427aa Protein coding CCDS4688 O75955-1 NM_005803.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
FLOT1-205 ENST00000438162.5 888 252aa Protein coding - A2AB10 - CDS 3' incomplete, TSL:5,
FLOT1-202 ENST00000413165.5 856 238aa Protein coding - A2AB09 - CDS 3' incomplete, TSL:5,
FLOT1-206 ENST00000445853.5 792 235aa Protein coding - A2AB12 - CDS 3' incomplete, TSL:1,
FLOT1-207 ENST00000454845.1 629 185aa Protein coding - A2AB13 - CDS 3' incomplete, TSL:3,
FLOT1-203 ENST00000416018.5 592 192aa Protein coding - A2AB11 - CDS 3' incomplete, TSL:3,
FLOT1-204 ENST00000418160.5 457 145aa Protein coding - A2ABJ5 - CDS 3' incomplete, TSL:3,
FLOT1-208 ENST00000470643.5 852 No protein Processed transcript - - - TSL:3,
FLOT1-212 ENST00000487376.5 1583 No protein Retained intron - - - TSL:5,
FLOT1-210 ENST00000484168.1 1101 No protein Retained intron - - - TSL:5,
FLOT1-211 ENST00000484693.1 1001 No protein Retained intron - - - TSL:2,
FLOT1-209 ENST00000476729.5 562 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FLOT1
This KO Strategy loading
Red Cotton™ Notes Gene FLOT1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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