CRISPR-U™ Gene Knockout Cell Line Strategy

HADHA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HADHA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HADHA
Gene id 3030
Organism Homo sapiens
Official full symbol hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
Gene type protein-coding
Summary This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Genomic regions Chromosome 2
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HADHA-201 ENST00000380649.8 2943 763aa Protein coding CCDS1721 P40939-1 NM_000182.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HADHA-204 ENST00000492433.2 2982 792aa Protein coding - H0YFD6 - TSL:2, GENCODE basic,
HADHA-209 ENST00000645274.1 2576 728aa Protein coding - A0A2R8Y4F5 - GENCODE basic,
HADHA-208 ENST00000644428.1 3087 499aa Nonsense mediated decay - A0A2R8YG21 - -
HADHA-205 ENST00000643057.1 2977 37aa Nonsense mediated decay - A0A2R8YDM1 - -
HADHA-206 ENST00000643063.1 2748 357aa Nonsense mediated decay - A0A2R8Y688 - -
HADHA-207 ENST00000643233.1 2688 37aa Nonsense mediated decay - A0A2R8YDM1 - -
HADHA-211 ENST00000646483.1 2158 82aa Nonsense mediated decay - P40939-2 - -
HADHA-210 ENST00000646031.1 1842 54aa Nonsense mediated decay - A0A2R8Y4E0 - CDS 5' incomplete,
HADHA-202 ENST00000461025.1 431 No protein Processed transcript - - - TSL:3,
HADHA-203 ENST00000471743.2 1485 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HADHA
This KO Strategy loading
Red Cotton™ Notes Gene HADHA had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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