CRISPR-U™ Gene Knockout Cell Line
Strategy
HADHA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HADHA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HADHA |
| Gene id | 3030 |
| Organism | Homo sapiens |
| Official full symbol | hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha |
| Gene type | protein-coding |
| Also known as | ECHA, GBP, HADH, LCEH, LCHAD, MTPA, TP-ALPHA |
| Summary | This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HADHA-201 | ENST00000380649.8 | 2943 | 763aa | Protein coding | CCDS1721 | P40939-1 | NM_000182.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HADHA-204 | ENST00000492433.2 | 2982 | 792aa | Protein coding | - | H0YFD6 | - | TSL:2, GENCODE basic, |
| HADHA-209 | ENST00000645274.1 | 2576 | 728aa | Protein coding | - | A0A2R8Y4F5 | - | GENCODE basic, |
| HADHA-208 | ENST00000644428.1 | 3087 | 499aa | Nonsense mediated decay | - | A0A2R8YG21 | - | - |
| HADHA-205 | ENST00000643057.1 | 2977 | 37aa | Nonsense mediated decay | - | A0A2R8YDM1 | - | - |
| HADHA-206 | ENST00000643063.1 | 2748 | 357aa | Nonsense mediated decay | - | A0A2R8Y688 | - | - |
| HADHA-207 | ENST00000643233.1 | 2688 | 37aa | Nonsense mediated decay | - | A0A2R8YDM1 | - | - |
| HADHA-211 | ENST00000646483.1 | 2158 | 82aa | Nonsense mediated decay | - | P40939-2 | - | - |
| HADHA-210 | ENST00000646031.1 | 1842 | 54aa | Nonsense mediated decay | - | A0A2R8Y4E0 | - | CDS 5' incomplete, |
| HADHA-202 | ENST00000461025.1 | 431 | No protein | Processed transcript | - | - | - | TSL:3, |
| HADHA-203 | ENST00000471743.2 | 1485 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HADHA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HADHA had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: