CRISPR-U™ Gene Knockout Cell Line
Strategy
EHMT2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EHMT2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EHMT2 |
| Gene id | 10919 |
| Organism | Homo sapiens |
| Official full symbol | euchromatic histone lysine methyltransferase 2 |
| Gene type | protein-coding |
| Also known as | BAT8, C6orf30, G9A, GAT8, KMT1C, NG36 |
| Summary | This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EHMT2-204 | ENST00000395728.7 | 4129 | 1267aa | Protein coding | CCDS87384 | A2ABF9 | - | TSL:1, GENCODE basic, APPRIS P4, |
| EHMT2-201 | ENST00000375528.8 | 4047 | 1233aa | Protein coding | CCDS75425 | A2ABF8 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| EHMT2-203 | ENST00000375537.8 | 3965 | 1210aa | Protein coding | CCDS4725 | Q96KQ7-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| EHMT2-202 | ENST00000375530.8 | 3856 | 1176aa | Protein coding | CCDS4726 | Q96KQ7-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| EHMT2-205 | ENST00000436026.1 | 894 | 298aa | Protein coding | - | H0Y4K5 | - | CDS 5' and 3' incomplete, TSL:5, |
| EHMT2-211 | ENST00000480912.5 | 3940 | No protein | Processed transcript | - | - | - | TSL:5, |
| EHMT2-210 | ENST00000478491.5 | 1517 | No protein | Processed transcript | - | - | - | TSL:5, |
| EHMT2-212 | ENST00000494816.5 | 3036 | No protein | Retained intron | - | - | - | TSL:5, |
| EHMT2-206 | ENST00000461880.5 | 1825 | No protein | Retained intron | - | - | - | TSL:5, |
| EHMT2-208 | ENST00000465429.1 | 1454 | No protein | Retained intron | - | - | - | TSL:2, |
| EHMT2-209 | ENST00000477678.1 | 679 | No protein | Retained intron | - | - | - | TSL:2, |
| EHMT2-207 | ENST00000463484.1 | 547 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EHMT2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EHMT2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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