CRISPR-U™ Gene Knockout Cell Line Strategy

EHMT2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EHMT2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EHMT2
Gene id 10919
Organism Homo sapiens
Official full symbol euchromatic histone lysine methyltransferase 2
Gene type protein-coding
Also known as BAT8, C6orf30, G9A, GAT8, KMT1C, NG36
Summary This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 6
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EHMT2-204 ENST00000395728.7 4129 1267aa Protein coding CCDS87384 A2ABF9 - TSL:1, GENCODE basic, APPRIS P4,
EHMT2-201 ENST00000375528.8 4047 1233aa Protein coding CCDS75425 A2ABF8 - TSL:2, GENCODE basic, APPRIS ALT2,
EHMT2-203 ENST00000375537.8 3965 1210aa Protein coding CCDS4725 Q96KQ7-1 - TSL:1, GENCODE basic, APPRIS ALT2,
EHMT2-202 ENST00000375530.8 3856 1176aa Protein coding CCDS4726 Q96KQ7-2 - TSL:5, GENCODE basic, APPRIS ALT2,
EHMT2-205 ENST00000436026.1 894 298aa Protein coding - H0Y4K5 - CDS 5' and 3' incomplete, TSL:5,
EHMT2-211 ENST00000480912.5 3940 No protein Processed transcript - - - TSL:5,
EHMT2-210 ENST00000478491.5 1517 No protein Processed transcript - - - TSL:5,
EHMT2-212 ENST00000494816.5 3036 No protein Retained intron - - - TSL:5,
EHMT2-206 ENST00000461880.5 1825 No protein Retained intron - - - TSL:5,
EHMT2-208 ENST00000465429.1 1454 No protein Retained intron - - - TSL:2,
EHMT2-209 ENST00000477678.1 679 No protein Retained intron - - - TSL:2,
EHMT2-207 ENST00000463484.1 547 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EHMT2
This KO Strategy loading
Red Cotton™ Notes Gene EHMT2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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