CRISPR-U™ Gene Knockout Cell Line Strategy

ITPA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ITPA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ITPA
Gene id 3704
Organism Homo sapiens
Official full symbol inosine triphosphatase
Gene type protein-coding
Also known as C20orf37, HLC14-06-P, ITPase, My049, NTPase, dJ794I6.3
Summary This gene encodes an inosine triphosphate pyrophosphohydrolase. The encoded protein hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. This protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency which causes an accumulation of ITP in red blood cells. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 20
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ITPA-201 ENST00000380113.8 1037 194aa Protein coding CCDS13051 Q9BY32-1 NM_033453.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ITPA-202 ENST00000399838.3 897 153aa Protein coding CCDS58762 Q9BY32-3 - TSL:1, GENCODE basic,
ITPA-203 ENST00000455664.6 729 177aa Protein coding CCDS46576 Q9BY32-2 - TSL:1, GENCODE basic,
ITPA-208 ENST00000483354.5 1028 No protein Processed transcript - - - TSL:3,
ITPA-209 ENST00000490838.6 977 No protein Processed transcript - - - TSL:2,
ITPA-204 ENST00000460550.5 967 No protein Processed transcript - - - TSL:2,
ITPA-207 ENST00000472295.1 670 No protein Processed transcript - - - TSL:2,
ITPA-205 ENST00000460676.5 488 No protein Processed transcript - - - TSL:3,
ITPA-206 ENST00000461029.1 378 No protein Processed transcript - - - TSL:5,
ITPA-210 ENST00000609835.5 924 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ITPA
This KO Strategy loading
Red Cotton™ Notes Gene ITPA had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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