CRISPR-U™ Gene Knockout Cell Line
Strategy
GLYR1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GLYR1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GLYR1 |
| Gene id | 84656 |
| Organism | Homo sapiens |
| Official full symbol | glyoxylate reductase 1 homolog |
| Gene type | protein-coding |
| Also known as | BM045, HIBDL, N-PAC, NP60, hNDF |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GLYR1-201 | ENST00000321919.14 | 3718 | 553aa | Protein coding | CCDS10524 | Q49A26-1 | NM_032569.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GLYR1-212 | ENST00000591451.5 | 1831 | 547aa | Protein coding | CCDS81945 | Q49A26-3 | - | TSL:1, GENCODE basic, |
| GLYR1-210 | ENST00000589389.5 | 3606 | 524aa | Protein coding | - | K7EMM8 | - | CDS 5' incomplete, TSL:1, |
| GLYR1-202 | ENST00000436648.9 | 3466 | 472aa | Protein coding | - | Q49A26-5 | - | TSL:2, GENCODE basic, |
| GLYR1-205 | ENST00000587297.1 | 688 | 171aa | Protein coding | - | K7EPU6 | - | CDS 5' incomplete, TSL:2, |
| GLYR1-208 | ENST00000588297.5 | 580 | 185aa | Protein coding | - | K7EQB2 | - | CDS 3' incomplete, TSL:3, |
| GLYR1-209 | ENST00000588732.5 | 3527 | 52aa | Nonsense mediated decay | - | K7ELL0 | - | TSL:1, |
| GLYR1-207 | ENST00000587936.5 | 897 | 37aa | Nonsense mediated decay | - | K7EK70 | - | CDS 5' incomplete, TSL:5, |
| GLYR1-204 | ENST00000586901.1 | 582 | No protein | Processed transcript | - | - | - | TSL:3, |
| GLYR1-206 | ENST00000587875.1 | 630 | No protein | Retained intron | - | - | - | TSL:2, |
| GLYR1-211 | ENST00000591159.1 | 557 | No protein | Retained intron | - | - | - | TSL:5, |
| GLYR1-213 | ENST00000591846.1 | 389 | No protein | Retained intron | - | - | - | TSL:3, |
| GLYR1-203 | ENST00000586095.1 | 319 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Levelloading
| Target Gene | GLYR1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GLYR1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Work flow
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