CRISPR-U™ Gene Knockout Cell Line Strategy

GLYR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GLYR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GLYR1
Gene id 84656
Organism Homo sapiens
Official full symbol glyoxylate reductase 1 homolog
Gene type protein-coding
Also known as BM045, HIBDL, N-PAC, NP60, hNDF
Genomic regions Chromosome 16
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GLYR1-201 ENST00000321919.14 3718 553aa Protein coding CCDS10524 Q49A26-1 NM_032569.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GLYR1-212 ENST00000591451.5 1831 547aa Protein coding CCDS81945 Q49A26-3 - TSL:1, GENCODE basic,
GLYR1-210 ENST00000589389.5 3606 524aa Protein coding - K7EMM8 - CDS 5' incomplete, TSL:1,
GLYR1-202 ENST00000436648.9 3466 472aa Protein coding - Q49A26-5 - TSL:2, GENCODE basic,
GLYR1-205 ENST00000587297.1 688 171aa Protein coding - K7EPU6 - CDS 5' incomplete, TSL:2,
GLYR1-208 ENST00000588297.5 580 185aa Protein coding - K7EQB2 - CDS 3' incomplete, TSL:3,
GLYR1-209 ENST00000588732.5 3527 52aa Nonsense mediated decay - K7ELL0 - TSL:1,
GLYR1-207 ENST00000587936.5 897 37aa Nonsense mediated decay - K7EK70 - CDS 5' incomplete, TSL:5,
GLYR1-204 ENST00000586901.1 582 No protein Processed transcript - - - TSL:3,
GLYR1-206 ENST00000587875.1 630 No protein Retained intron - - - TSL:2,
GLYR1-211 ENST00000591159.1 557 No protein Retained intron - - - TSL:5,
GLYR1-213 ENST00000591846.1 389 No protein Retained intron - - - TSL:3,
GLYR1-203 ENST00000586095.1 319 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GLYR1
This KO Strategy loading
Red Cotton™ Notes Gene GLYR1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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