CRISPR-U™ Gene Knockout Cell Line
Strategy
CRKL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CRKL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CRKL |
| Gene id | 1399 |
| Organism | Homo sapiens |
| Official full symbol | CRK like proto-oncogene, adaptor protein |
| Gene type | protein-coding |
| Summary | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CRKL-201 | ENST00000354336.8 | 5342 | 303aa | Protein coding | CCDS13785 | P46109 | NM_005207.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CRKL-202 | ENST00000411769.1 | 2037 | 303aa | Nonsense mediated decay | CCDS13785 | P46109 | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CRKL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CRKL had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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