CRISPR-U™ Gene Knockout Cell Line Strategy

HNRNPDL Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HNRNPDL Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HNRNPDL
Gene id 9987
Organism Homo sapiens
Official full symbol heterogeneous nuclear ribonucleoprotein D like
Gene type protein-coding
Summary This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind to RNAs. Three alternatively spliced transcript variants have been described for this gene. One of the variants is probably not translated because the transcript is a candidate for nonsense-mediated mRNA decay. The protein isoforms encoded by this gene are similar to its family member HNRPD.
Genomic regions Chromosome 4
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HNRNPDL-201 ENST00000295470.10 4372 420aa Protein coding CCDS3593 O14979-1 NM_031372.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
HNRNPDL-208 ENST00000621267.4 4139 420aa Protein coding CCDS3593 O14979-1 - TSL:1, GENCODE basic, APPRIS P4,
HNRNPDL-207 ENST00000614627.4 3968 363aa Protein coding CCDS75153 A0A087WUK2 - TSL:1, GENCODE basic, APPRIS ALT1,
HNRNPDL-204 ENST00000507721.5 2404 301aa Protein coding - O14979-2 - TSL:1, GENCODE basic,
HNRNPDL-210 ENST00000630827.1 1318 301aa Protein coding - O14979-2 - TSL:5, GENCODE basic,
HNRNPDL-206 ENST00000602300.5 1291 301aa Protein coding - O14979-2 - TSL:1, GENCODE basic,
HNRNPDL-203 ENST00000502762.4 2356 420aa Nonsense mediated decay CCDS3593 O14979-1 - TSL:1,
HNRNPDL-209 ENST00000630114.2 1433 301aa Nonsense mediated decay - O14979-2 - TSL:5,
HNRNPDL-202 ENST00000349655.8 1402 301aa Nonsense mediated decay - O14979-2 - TSL:1,
HNRNPDL-205 ENST00000514511.1 1143 57aa Nonsense mediated decay - A0A087WU03 - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HNRNPDL
This KO Strategy loading
Red Cotton™ Notes Gene HNRNPDL had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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