CRISPR-U™ Gene Knockout Cell Line Strategy

NUP153 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NUP153 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NUP153
Gene id 9972
Organism Homo sapiens
Official full symbol nucleoporin 153
Gene type protein-coding
Also known as HNUP153, N153
Summary Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NUP153-202 ENST00000537253.5 6030 1506aa Protein coding CCDS64359 P49790-3 - TSL:2, GENCODE basic, APPRIS ALT2,
NUP153-201 ENST00000262077.3 6026 1475aa Protein coding CCDS4541 P49790-1 NM_005124.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
NUP153-203 ENST00000613258.4 5811 1433aa Protein coding CCDS75407 P49790-2 - TSL:1, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NUP153
This KO Strategy loading
Red Cotton™ Notes Gene NUP153 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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