CRISPR-U™ Gene Knockout Cell Line
Strategy
GOLGA5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GOLGA5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GOLGA5 |
| Gene id | 9950 |
| Organism | Homo sapiens |
| Official full symbol | golgin A5 |
| Gene type | protein-coding |
| Also known as | GOLIM5, RFG5, ret-II |
| Summary | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GOLGA5-201 | ENST00000163416.7 | 2879 | 731aa | Protein coding | CCDS9905 | Q8TBA6-1 | NM_005113.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GOLGA5-202 | ENST00000554700.1 | 759 | 117aa | Protein coding | - | H0YK03 | - | CDS 5' incomplete, TSL:1, |
| GOLGA5-203 | ENST00000555793.1 | 586 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GOLGA5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GOLGA5 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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