CRISPR-U™ Gene Knockout Cell Line Strategy

LPGAT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LPGAT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LPGAT1
Gene id 9926
Organism Homo sapiens
Official full symbol lysophosphatidylglycerol acyltransferase 1
Gene type protein-coding
Also known as FAM34A, FAM34A1, NET8
Summary This gene encodes a member of the lysophospholipid acyltransferase family. The encoded protein catalyzes the reacylation of lysophosphatidylglycerol to phosphatidylglycerol, a membrane phospholipid that is an important precursor for the synthesis of cardiolipin. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LPGAT1-202 ENST00000366997.9 7773 370aa Protein coding CCDS31018 Q92604 NM_014873.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LPGAT1-201 ENST00000366996.1 2035 370aa Protein coding CCDS31018 Q92604 - TSL:1, GENCODE basic, APPRIS P1,
LPGAT1-203 ENST00000488600.1 439 No protein Processed transcript - - - TSL:3,
LPGAT1-204 ENST00000498690.1 300 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LPGAT1
This KO Strategy loading
Red Cotton™ Notes Gene LPGAT1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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