CRISPR-U™ Gene Knockout Cell Line
Strategy
LPGAT1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LPGAT1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LPGAT1 |
| Gene id | 9926 |
| Organism | Homo sapiens |
| Official full symbol | lysophosphatidylglycerol acyltransferase 1 |
| Gene type | protein-coding |
| Also known as | FAM34A, FAM34A1, NET8 |
| Summary | This gene encodes a member of the lysophospholipid acyltransferase family. The encoded protein catalyzes the reacylation of lysophosphatidylglycerol to phosphatidylglycerol, a membrane phospholipid that is an important precursor for the synthesis of cardiolipin. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LPGAT1-202 | ENST00000366997.9 | 7773 | 370aa | Protein coding | CCDS31018 | Q92604 | NM_014873.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LPGAT1-201 | ENST00000366996.1 | 2035 | 370aa | Protein coding | CCDS31018 | Q92604 | - | TSL:1, GENCODE basic, APPRIS P1, |
| LPGAT1-203 | ENST00000488600.1 | 439 | No protein | Processed transcript | - | - | - | TSL:3, |
| LPGAT1-204 | ENST00000498690.1 | 300 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LPGAT1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LPGAT1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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