CRISPR-U™ Gene Knockout Cell Line Strategy

FAM20B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FAM20B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FAM20B
Gene id 9917
Organism Homo sapiens
Official full symbol FAM20B glycosaminoglycan xylosylkinase
Gene type protein-coding
Also known as gxk1
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FAM20B-201 ENST00000263733.5 5991 409aa Protein coding CCDS1328 A0A024R918 O75063 NM_014864.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
FAM20B-202 ENST00000440702.5 603 138aa Protein coding - X6RH03 - CDS 3' incomplete, TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FAM20B
This KO Strategy loading
Red Cotton™ Notes Gene FAM20B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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