CRISPR-U™ Gene Knockout Cell Line
Strategy
DENND4B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DENND4B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DENND4B |
| Gene id | 9909 |
| Organism | Homo sapiens |
| Official full symbol | DENN domain containing 4B |
| Gene type | protein-coding |
| Also known as | KIAA0476 |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DENND4B-201 | ENST00000361217.9 | 5728 | 1496aa | Protein coding | CCDS44228 | O75064 | NM_014856.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DENND4B-202 | ENST00000368646.6 | 3781 | 1218aa | Protein coding | - | E9PAK5 | - | CDS 3' incomplete, TSL:5, |
| DENND4B-205 | ENST00000472932.2 | 541 | 181aa | Protein coding | - | H0YEF0 | - | CDS 5' and 3' incomplete, TSL:5, |
| DENND4B-206 | ENST00000474386.1 | 455 | No protein | Processed transcript | - | - | - | TSL:3, |
| DENND4B-208 | ENST00000480340.1 | 2530 | No protein | Retained intron | - | - | - | TSL:2, |
| DENND4B-211 | ENST00000492898.1 | 951 | No protein | Retained intron | - | - | - | TSL:5, |
| DENND4B-210 | ENST00000485359.1 | 891 | No protein | Retained intron | - | - | - | TSL:2, |
| DENND4B-203 | ENST00000462423.1 | 783 | No protein | Retained intron | - | - | - | TSL:4, |
| DENND4B-204 | ENST00000464048.1 | 551 | No protein | Retained intron | - | - | - | TSL:2, |
| DENND4B-213 | ENST00000531748.1 | 544 | No protein | Retained intron | - | - | - | TSL:4, |
| DENND4B-209 | ENST00000483561.2 | 499 | No protein | Retained intron | - | - | - | TSL:4, |
| DENND4B-207 | ENST00000477746.1 | 457 | No protein | Retained intron | - | - | - | TSL:2, |
| DENND4B-212 | ENST00000494683.1 | 456 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DENND4B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DENND4B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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