CRISPR-U™ Gene Knockout Cell Line Strategy

DENND4B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DENND4B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DENND4B
Gene id 9909
Organism Homo sapiens
Official full symbol DENN domain containing 4B
Gene type protein-coding
Also known as KIAA0476
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DENND4B-201 ENST00000361217.9 5728 1496aa Protein coding CCDS44228 O75064 NM_014856.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DENND4B-202 ENST00000368646.6 3781 1218aa Protein coding - E9PAK5 - CDS 3' incomplete, TSL:5,
DENND4B-205 ENST00000472932.2 541 181aa Protein coding - H0YEF0 - CDS 5' and 3' incomplete, TSL:5,
DENND4B-206 ENST00000474386.1 455 No protein Processed transcript - - - TSL:3,
DENND4B-208 ENST00000480340.1 2530 No protein Retained intron - - - TSL:2,
DENND4B-211 ENST00000492898.1 951 No protein Retained intron - - - TSL:5,
DENND4B-210 ENST00000485359.1 891 No protein Retained intron - - - TSL:2,
DENND4B-203 ENST00000462423.1 783 No protein Retained intron - - - TSL:4,
DENND4B-204 ENST00000464048.1 551 No protein Retained intron - - - TSL:2,
DENND4B-213 ENST00000531748.1 544 No protein Retained intron - - - TSL:4,
DENND4B-209 ENST00000483561.2 499 No protein Retained intron - - - TSL:4,
DENND4B-207 ENST00000477746.1 457 No protein Retained intron - - - TSL:2,
DENND4B-212 ENST00000494683.1 456 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DENND4B
This KO Strategy loading
Red Cotton™ Notes Gene DENND4B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×