CRISPR-U™ Gene Knockout Cell Line
Strategy
TLK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TLK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TLK1 |
| Gene id | 9874 |
| Organism | Homo sapiens |
| Official full symbol | tousled like kinase 1 |
| Gene type | protein-coding |
| Also known as | PKU-beta |
| Summary | The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TLK1-205 | ENST00000431350.7 | 5723 | 766aa | Protein coding | CCDS2241 | Q9UKI8 | NM_012290.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| TLK1-212 | ENST00000521943.5 | 2605 | 718aa | Protein coding | CCDS46447 | - | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| TLK1-206 | ENST00000434911.6 | 2156 | 670aa | Protein coding | CCDS46448 | - | - | TSL:2, GENCODE basic, |
| TLK1-202 | ENST00000360843.7 | 5726 | 787aa | Protein coding | - | - | - | TSL:1, GENCODE basic, |
| TLK1-207 | ENST00000453628.1 | 539 | 162aa | Protein coding | - | C9JWT6 | - | CDS 3' incomplete, TSL:4, |
| TLK1-201 | ENST00000359766.7 | 4321 | 116aa | Nonsense mediated decay | - | F8W7X8 | - | TSL:5, |
| TLK1-203 | ENST00000409443.6 | 2783 | 413aa | Nonsense mediated decay | - | B3KRP1 | - | TSL:2, |
| TLK1-211 | ENST00000486857.5 | 715 | No protein | Processed transcript | - | - | - | TSL:3, |
| TLK1-204 | ENST00000413010.5 | 603 | No protein | Processed transcript | - | - | - | TSL:5, |
| TLK1-209 | ENST00000470340.1 | 521 | No protein | Processed transcript | - | - | - | TSL:4, |
| TLK1-208 | ENST00000466220.1 | 346 | No protein | Processed transcript | - | - | - | TSL:4, |
| TLK1-210 | ENST00000478683.1 | 2749 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TLK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TLK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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