CRISPR-U™ Gene Knockout Cell Line Strategy

TLK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TLK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TLK1
Gene id 9874
Organism Homo sapiens
Official full symbol tousled like kinase 1
Gene type protein-coding
Also known as PKU-beta
Summary The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TLK1-205 ENST00000431350.7 5723 766aa Protein coding CCDS2241 Q9UKI8 NM_012290.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
TLK1-212 ENST00000521943.5 2605 718aa Protein coding CCDS46447 - - TSL:2, GENCODE basic, APPRIS ALT1,
TLK1-206 ENST00000434911.6 2156 670aa Protein coding CCDS46448 - - TSL:2, GENCODE basic,
TLK1-202 ENST00000360843.7 5726 787aa Protein coding - - - TSL:1, GENCODE basic,
TLK1-207 ENST00000453628.1 539 162aa Protein coding - C9JWT6 - CDS 3' incomplete, TSL:4,
TLK1-201 ENST00000359766.7 4321 116aa Nonsense mediated decay - F8W7X8 - TSL:5,
TLK1-203 ENST00000409443.6 2783 413aa Nonsense mediated decay - B3KRP1 - TSL:2,
TLK1-211 ENST00000486857.5 715 No protein Processed transcript - - - TSL:3,
TLK1-204 ENST00000413010.5 603 No protein Processed transcript - - - TSL:5,
TLK1-209 ENST00000470340.1 521 No protein Processed transcript - - - TSL:4,
TLK1-208 ENST00000466220.1 346 No protein Processed transcript - - - TSL:4,
TLK1-210 ENST00000478683.1 2749 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TLK1
This KO Strategy loading
Red Cotton™ Notes Gene TLK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×